| GUO Zhe,LV Qiu-jun,GUO Jian-ping.Study on radiation protection of Pueraria Flavonid and its molecular mechanism[J].Chinese Journal of Radiological Medicine and Protection,2006,26(6):579-582 |
| Study on radiation protection of Pueraria Flavonid and its molecular mechanism |
| Received:January 20, 2006 |
| DOI： |
| KeyWords:Pueraria Flavonid Radiation Mouse Apoptosis |
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| Abstract:: |
| Objective To investigate radiation protection of Pueraria Flavonid and its molecular mechanism. Methods Kunming mice were intragastrically (ig) administered with Pueraria Flavonid before exposure to 60Co γ-ray radiation of 5 Gy. Thirty day survival and average life span of dead mice after irradiation were estimated. Peripheral blood parameters, the organ indexes of thymus and spleen and antioxidant activity were also observed in the irradiated mice. The apoptosis of spleen cells in irradiated mice was detected by FACS and in situ terminal labeling method. The effect of Pueraria Flavonid on the activities of caspase-3 in spleen cells were examined. Results Administration with Pueraria Flavonid increased the 30-day survival and prolonged the average life span of the dead mice. The increases in the number of white blood cells and the organ indexes of thymus and spleen, the decreases in apoptosis rates and caspase-3 activities in spleen cells were observed in Pueraria Flavonid treated group. Cu/Zn-SOD activity was increased and MDA levels significant decreased with administration of Pueraria Flavonid. Conclusions Pueraria Flavonid performs function in radiation protection, and the underlying mechanism might be related with suppression of apoptosis and antioxidant activity in radiation-sensitive cells. |
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