| LIU Qiang,JIANG En-hai,LI Jin.Study on dose-effect relationship of radiation-induced DNA damage using single cell gel electrophoresis[J].Chinese Journal of Radiological Medicine and Protection,2006,26(6):573-576 |
| Study on dose-effect relationship of radiation-induced DNA damage using single cell gel electrophoresis |
| Received:January 10, 2006 |
| DOI： |
| KeyWords:Single cell gel electrophoresis Radiation biodosimetry DNA double-strand break Radiation accident |
| FundProject:教育部辐射血液学重点学科基金资助;天津市自然科学基金资助项目(043610011) |
| Author Name | Affiliation | E-mail | | LIU Qiang | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China | jnh1953@yahoo.com.cn | | JIANG En-hai | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China | | | LI Jin | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China | | | 唐卫生 | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China | | | 王知权 | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China | | | 赵永成 | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China | | | 樊飞跃 | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China | |
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| Abstract:: |
| Objective To explore a new, simple and quick radiation biodosimeter, which can be applied to estimate biological dose in case of radiation accident. Methods DNA double-strand break were detected using neutral single cell gel electrophoresis (SCGE), and all the indexes of comet assay including HDNA%, TDNA%, CL, TL, TM, and OTM were analyzed by CASP (Comet Assay Software Project). The curve of dose-effect was fitted using SPSS 12.0 software. Results Statistically significant dose-effect relationships were observed in all the indexes of comet assay, OTM was superior to other indexes. Conclusions Application of neutral comet assay combined with CASP analysis could serve as a new radiation biodosimeter. |
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