| SHEN Bo,JU Gui-zhi,LIU Yang.Expressions of survivin and P53 and their correlation in EL-4 cell line[J].Chinese Journal of Radiological Medicine and Protection,2006,26(6):563-565 |
| Expressions of survivin and P53 and their correlation in EL-4 cell line |
| Received:December 13, 2005 |
| DOI： |
| KeyWords:Survivin P53 Apoptosis cell |
| FundProject:国家自然科学基金资助项目(30270346) |
| Author Name | Affiliation | E-mail | | SHEN Bo | MH Key Radiobiology Unit, School of Public Health, Jilin University, Changchun 130021, China | jugz@jlu.edu.cn | | JU Gui-zhi | MH Key Radiobiology Unit, School of Public Health, Jilin University, Changchun 130021, China | | | LIU Yang | MH Key Radiobiology Unit, School of Public Health, Jilin University, Changchun 130021, China | | | 冯天水 | MH Key Radiobiology Unit, School of Public Health, Jilin University, Changchun 130021, China | | | 李乐 | MH Key Radiobiology Unit, School of Public Health, Jilin University, Changchun 130021, China | |
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| Abstract:: |
| Objective To explore the expression of survivin, an anti-apoptosis gene, in EL-4 cell line and its relationship with the expression of P53. Methods EL-4 cells were irradiated with 4 Gy X-rays to induce the expressions of survivin and P53. Flow cytometry assay was used to detect the expression of survivin and P53 and incidence of apoptosis following the irradiation. Results X-ray irradiation of 4 Gy down-regulated the expression of survivin in EL-4 cells while the expression of P53 was upregulated. A statistically lower expression of survivin was observed at 4 h after the irradiation when compared with the control group (P<0.001), while the expression of P53 was significantly increased in the exposed group (P<0.01). The incidence of apoptosis in the exposed group were significantly higher than that in the control group(P<0.001). Conclusions It is shown that survivin and P53 play important roles in regulating the cell apoptosis, and suggested that a possible correlation exists between expressions of survivin and P53. |
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