| JU Gui-zhi,SHEN Bo,SUN Shi-long.Expression of Caspase-3,P53 in EL-4 cells induced by ionizing radiation and its biological implications[J].Chinese Journal of Radiological Medicine and Protection,2006,26(6):560-562 |
| Expression of Caspase-3,P53 in EL-4 cells induced by ionizing radiation and its biological implications |
| Received:January 10, 2006 |
| DOI： |
| KeyWords:Ionizing radiation Caspase-3 P53 Apoptosis Polyploid |
| FundProject:国家自然科学基金资助项目(30270346) |
| Author Name | Affiliation | E-mail | | JU Gui-zhi | MH Key Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | jugz@jlu.edu.cn | | SHEN Bo | MH Key Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | | | SUN Shi-long | MH Key Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | | | 闫凤琴 | MH Key Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | | | 付士波 | MH Key Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | | | 李鹏武 | MH Key Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, China | |
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| Abstract:: |
| Objective To investigate the effect of ionizing radiation on the expressions of Caspase-3 and P53 proteins in EL-4 cells and its implications in the induction of apoptosis and polyploid cells. Methods EL-4 cells were irradiated with 4.0 Gy X-rays (180 kV, 15 mA, 0.287 Gy/min). Fluorescent staining and flow cytometry analysis were used to measure protein expression, apoptosis and polyploid cells. Results It was found that the expression of Caspase-3 protein was increased significantly at 8 h and 12 h after the irradiation compared with sham-irradiated control (P<0.05), and the expression of P53 protein was also increased significantly at 2,4,8,12 and 24 h after the irradiation compared with sham-irradiated control (P< 0.05 or P<0.01). The results showed that apoptosis of EL-4 cells was increased significantly at 2,4,8,12,24,48, and 72 h after 4.0 Gy irradiation compared with sham-irradiated control (P<0.05 or P<0.01 or P<0.001). However, no significant change in the number of polyploidy cells was found during the period from 2 to 48 h after the irradiation with 4.0 Gy X-rays. Conclusions It is indicated that the expressions of Caspase-3 and P53 protein in EL-4 cells can be induced by ionizing radiation, and play an important role in the induction of apoptosis; the molecular pathway for polyploid formation might be P53-independent. |
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