| ZHANG Ke-jie,LIU Wen-li,FU Jin-rong.Expression of Notch in murine bone marrow stromal cells with radiation injury and its significance[J].Chinese Journal of Radiological Medicine and Protection,2006,26(6):557-559 |
| Expression of Notch in murine bone marrow stromal cells with radiation injury and its significance |
| Received:December 02, 2005 |
| DOI： |
| KeyWords:Radiation injury Bone marrow stromal cells Notch signaling |
| FundProject:国家自然科学基金资助项目(30570773) |
| Author Name | Affiliation | E-mail | | ZHANG Ke-jie | Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | liuwenli1945@sina.com.cn | | LIU Wen-li | Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | | FU Jin-rong | Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | | 朱艳 | Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | | 黄丽芳 | Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | | 孙汉英 | Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | | | 周剑峰 | Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China | |
|
| Hits: 3106 |
| Download times: 2052 |
| Abstract:: |
| Objective To investigate the change of Notch expression in murine bone marrow stromal cell after exposure to γ-rays in vitro. Methods Cultured murine bone marrow stromal cells irradiated with 8.0 Gy 60Co γ-rays were used in this study. The Notch1, Jagged1, Hes1 genes expressions were analyzed by RT-PCR at 2 h, 4 h, 8 h, and 12 h after the irradiation. Results There were no expression of Notch1, Jagged1, and Hes1 genes in normal murine bone marrow stromal cells at mRNA level, while Notch1, Jagged1, and Hes1 genes were expressed at 2 h after the irradiation, and reached a peak at 4 h, then returned back to the normal level at 8-12 h. The differences between the exposed group and the control group were statistically significant (P<0.001). Conclusions Notch signaling pathway was activated in cultured murine bone marrow stromal cellls after exposure to γ-rays, it is suggested that Notch signaling pathway is involved in the radiation damage to murine bone marrow stromal cells. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
