| SONG Liang-wen,SUN Li,DIAO Rui_ying.Effect of macrophage and matrix metalloproteinase-9 on proliferation of pulmonary fibroblast and synthesis of collagen Ⅳ[J].Chinese Journal of Radiological Medicine and Protection,2006,26(6):549-552 |
| Effect of macrophage and matrix metalloproteinase-9 on proliferation of pulmonary fibroblast and synthesis of collagen Ⅳ |
| Received:December 07, 2005 |
| DOI： |
| KeyWords:Radiation pulmonary injury Alveolar macrophage Fibroblast Type Ⅳ Collagen Matrix metalloproteinase-9 |
| FundProject:国家自然科学基金资助项目(30570545) |
| Author Name | Affiliation | E-mail | | SONG Liang-wen | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | Lsong1@Yahoo.com | | SUN Li | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | DIAO Rui_ying | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 李杨 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 张勇 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 尹纪业 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | |
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| Abstract:: |
| Objective To explore pathogenetic mechanism in initiation of radiation-induced pulmonary fibrosis. Methods Alveolar macrophages in Wistar rats irradiated by 60Co γ-ray were collected by alveolar lavage; condition medium was prepared for stimulating human lung fibroblast (HLF) proliferation; HLF proliferation activity was determined by MTT method; collagen Ⅳ (Col Ⅳ) in HLF was determined by Western blot; the activity of matrix metalloproteinase-9 (MMP-9) was determined by zymography. Results HLF proliferation activity was significantly increased after stimulation of condition medium, and the increase was most evident within 48-72 hs. Col Ⅳ synthesis in HLF was increased and reached a peak at 12 h after stimulation and then began to decrease. MMP-9 activity began to increase at 12 h and reached a peak at 48 h and then decreased after 72 h. Conclusions Cobalt-60 gamma ray irradiation of 20 Gy can stimulate secretion of some cytokines in alveolar macrophage to promote pulmonary interstitial fibroblast proliferation and synthesis of Col Ⅳ. Col Ⅳ can stimulate MMP-9 increase; MMP-9 can degrade excess Col Ⅳ. Such changes are involved in remodeling process of early pulmonary injury. |
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