| LI Lin-fa,WEI Dong,ZHANG Jun.Using dynamic bone imaging with 99mTc-MDP for quantitatively evaluating the repairing of skull defect with tissue-engineered bone graft in rabbits[J].Chinese Journal of Radiological Medicine and Protection,2006,26(5):498-500 |
| Using dynamic bone imaging with 99mTc-MDP for quantitatively evaluating the repairing of skull defect with tissue-engineered bone graft in rabbits |
| Received:December 07, 2005 |
| DOI： |
| KeyWords:Dynamic bone imaging 99mTc -MDP Tissue-engineered bone Bone remodeling |
| FundProject: |
| Author Name | Affiliation | E-mail | | LI Lin-fa | Department of Nuclear Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China | wangmysm@yahoo.com | | WEI Dong | 310003 杭州, 浙江大学医学院附属第一医院口腔科 | | | ZHANG Jun | Department of Nuclear Medicine, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China | | | 王慧明 | 310003 杭州, 浙江大学医学院附属第一医院口腔科 | |
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| Abstract:: |
| Objective To investigate the practical value of dynamic bone imaging with 99mTc -MDP in quantitatively evaluating the vascularization and ossification effects during the repairing of bone defect with the tissue-engineered bone graft. Methods 15 model rabbits of skull defect were built which were symmetrically implanted nano-hydroxyapatite/collagen-1-type (nHAC) and particle-hydroxyapatite/collagen-1-type (pHAC) respectively into the defect sites. They were randomly and averagely divided into 5 time-point groups, namely 2, 4, 8, 10 and 12 week groups. After intravenous injection of 99mTc -MDP via the auricle vein,the routinely dynamic bone imaging was performed. Using the ROI technique, the radioactivity peak and uptake ratio were calculated. The parameters mentioned obove were compared between the nHAC and pHAC. The X-ray of skull was done and the images were observed. Results (1) Dynamic bone imaging: at every time-point, the radioactivity peak of perfusion phase and uptake ratio of static phase in nHAC implanted area both were significantly higher than those in pHAC implanted area (P<0.01). With the implantation time extending, the radioactivity peak of perfusion phase and uptake ratio of static phase in nHAC and pHAC implanted areas both increased; and these parameters after 8 week were significantly higher than those at 2 week in nHAC implanted area (P<0.05), while after 10 weeks in pHAC implanted area (P<0.01). (2) X-ray imaging: The bone repairing of nHAC was earlier than pHAC, and the density of neo-bone in nHAC was also greater than that in pHAC. Both edge and center of implanted area presented neo-born in nHAC, but only edge in pHAC. Conclusions In promoting early vascularization and accelerating ossification, the nHAC has obvious superiority to the pHAC. The dynamic bone imaging with 99mTc -MDP may evaluate the vascularization and ossification effects accurately, dynamically and quantitatively during the repairing of bone defect with the tissue-engineered bone graft. |
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