| YANG Kun-yu,HU Yu,Ruebe Claudia.Expressions of TNF-α, IL-1α and IL-6 in bronchiolar epithelium after thoracic irradiation[J].Chinese Journal of Radiological Medicine and Protection,2006,26(5):483-485 |
| Expressions of TNF-α, IL-1α and IL-6 in bronchiolar epithelium after thoracic irradiation |
| Received:April 30, 2006 |
| DOI： |
| KeyWords:Radiation-induced lung injury Proinflammatory cytokines X rays |
| FundProject: |
| Author Name | Affiliation | E-mail | | YANG Kun-yu | Wuhan Union Hospital Cancer Center, Tongji Medical college, Huazhong Science and Technology University, Wuhan 430023, China | huyu1964@163.net | | HU Yu | 430023 武汉, 华中科技大学同济医学院附属协和医院血液学研究所 | | | Ruebe Claudia | Department of RadiationOncology, Saarland University Hospital, Hamburg/Saar 66424, Germany | | | Ruebe Christian | Department of RadiationOncology, Saarland University Hospital, Hamburg/Saar 66424, Germany | |
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| Abstract:: |
| Objective To investigate temporal and spatial releases of proinflam matory cytokines TNF-α, IL-1α and IL-6 in the lung tissue after thoracic irradiation in C57BL/6J mice. Methods Mice were irradiated with 12 Gy to whole lungs, and control mice were sham-irradiated. Mice were sacrificed at hours 0.5, 1, 3, 6, 12, 24, 48 and 72 and weeks 1,2, 4, 8, 16 and 24 after thoracic irradiation or sham-irradiation. Expressions of TNF-α and IL-1α, IL-6 proteins were detected with immuno-histochemistry. Results At hour 6 after thoracic irradiation, the expression of TNF-α protein increased significantly, and at hour 12 after thoracic irradiation, the expressions of IL-1α and IL-6 proteins increased significantly, too. The bronchiolar epithelium was the most prominent source of these inflammatory cytokines in the first hours. During the stage of acute pneumonitis, the bronchiolar epithelium, as well as inflammatory cells in the lung interstitium, produced high amounts of TNF-α, IL-1α and IL-6. Conclusions It was demonstrated that immediate expressions of TNF-α, IL-1α and IL-6 occurred in the bronchiolar epithelium after lung irradiation, and a long-lasting release by the bronchiolar and alveolar epithelium, and inflammatory cells during acute pneumonitis. Therefore, the bronchiolar epithelium is a significant source of proinflammatory cytokines capable of promoting inflammation through recruitment and activation of inflammatory cells after lung irradiation. |
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