| WANG Zhi-dong,CHEN Xiao-hua,DONG Bo.Expression of haptoglobin induced by γ-ray irradiation in THP-1 cell line[J].Chinese Journal of Radiological Medicine and Protection,2006,26(5):463-467 |
| Expression of haptoglobin induced by γ-ray irradiation in THP-1 cell line |
| Received:October 25, 2005 |
| DOI： |
| KeyWords:γ-ray Haptoglobin C/EBPs |
| FundProject: |
| Author Name | Affiliation | E-mail | | WANG Zhi-dong | Beijing Institute of Radiation Medicine, Beijing 100850, China | chenxh@nic.bmi.ac.cn | | CHEN Xiao-hua | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | DONG Bo | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 侯丽莉 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 张军权 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 饶亚岚 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 高荣莲 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 丛悦 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 毛秉智 | Beijing Institute of Radiation Medicine, Beijing 100850, China | |
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| Abstract:: |
| Objective To study the induction and regulation mechanisms for haptoglobin induced by γ-ray irradiation in THP-1 cell line. Methods The mRNA and protein of haptoglobin or C/EBPs were detected by RT-PCR and Western blot respectively. EMSA was used to determine the compounds of nuclear extracts and DNA probe which included the sequence of haptoglobin gene promoter. Super shift assay was also applied to analyze the transcription factors that may participate in the formation of compounds. Results γ-ray irradiation induced the synthesis of haptoglobin, which showed a dose-and time-dependence. Western blot analysis showed that γ-ray irradiation increased Stat3 expression, but reduced expression of C/EBPβ-LIP. EMSA and super shift assay demonstrated that Stat3, C/EBPα, and C/EBPβ were bound to probe of promoter. Conclusions γ-rays can induce haptoglobin expression in THP-1 cell line. Stat3 and C/EBPβ-LIP are involved in the activation and regulation of haptoglobin gene expression. |
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