| ZOU Yue,LI Zhi_ren,ZHOU Xiang-yan.Effect of RNAi inhibiting telomerase on telomere lengths after irradiation[J].Chinese Journal of Radiological Medicine and Protection,2006,26(5):453-455 |
| Effect of RNAi inhibiting telomerase on telomere lengths after irradiation |
| Received:October 27, 2005 |
| DOI： |
| KeyWords:RNAi Telomerase Telomere Radiation |
| FundProject:全军“十五”卫生科研规划资助项目(01MB054) |
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| Abstract:: |
| Objective To investigate the effect of expression vector of RNAi on inhibiting expression of telomerase, and its influence on telomere lengths in human cell line exposed to ionizing radiation. Methods Expression vector of pPUR/hTERT interfering with hTERT mRNA was constructed by inserting oligodeoxynucleotide into pPUR/U6 vector. Transfecied A549 line was mediated by Lipofectamine. Telomerase activities were measured by TRAP-ELISA, expression of hTERT mRNA by RT-PCR, and telomere lengths by mean length of terminal restriction fragment (TRF) analysis. Results An expression of the vector inhibited telomerase activities in transferred A549 and the expression of hTERT mRNA was down-regulated. After irradiation with coblat-60 γ-rays, length of telomere of A549 increased significantly, however, telomere lengthening did not occur in pPUR/hTERT line. Conclusions The expression vector of RNAi could inhibit expression of telomerase and telomere lengthening after radiation, suggesting that telomere lengthening induced by irradiation may be attributed to telomerase de novo composition of telomere sequences. |
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