| GAO Rong-lian,Dong Bo,RAO Ya-lan.Preliminary research on death pattern in PC12 cell after high-dose of gamma-ray irradiation[J].Chinese Journal of Radiological Medicine and Protection,2006,26(5):449-452 |
| Preliminary research on death pattern in PC12 cell after high-dose of gamma-ray irradiation |
| Received:October 28, 2005 |
| DOI： |
| KeyWords:Ionizing radiation PC12 cell Oncosis |
| FundProject: |
| Author Name | Affiliation | E-mail | | GAO Rong-lian | Beijing Institute of Radiation Medicine, Beijing 100850, China | chenxh@nic.bmi.ac.cn | | Dong Bo | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | RAO Ya-lan | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 陈肖华 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 张军权 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 王治东 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 侯丽莉 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 毛秉智 | Beijing Institute of Radiation Medicine, Beijing 100850, China | |
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| Abstract:: |
| Objective To investigate the death pattern of pheochromocyoma cell line (PC12 cell) irradiated with ionizing radiation and to search for a model of radiation-induced neuron injury in vitro. Methods PC12 cell was irradiated with different doses of 60Co γ-rays. The effect of radiation on cell cycle, development, differentiation and death rate were detected by flow cytometry, light microscope, and transmission electron microscope (TEM). Results Irradiated PC12 cell showed cell cycle retardation and differentiation, but some cells died due to swelling. Apoptosis was observed in few cells. TEM examination indicated that mitochondrion and endoplasmic reticulum were dilated markedly, chromatin was concentrated and scattered near karyotheca or around the nucleolus, which characterized oncosis. Conclusions PC12 cell died through oncosis after gamma-ray irradiation of high-dose, and can be used as a radiation-induced neuron injury model. |
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