| LIU Qing-jie,LU Xue,FENG Jiang-bin.Preliminary study on biological dosimetry using alkaline single cell gel electrophoresis of human peripheral lymphocytes[J].Chinese Journal of Radiological Medicine and Protection,2006,26(5):446-448 |
| Preliminary study on biological dosimetry using alkaline single cell gel electrophoresis of human peripheral lymphocytes |
| Received:November 15, 2005 |
| DOI： |
| KeyWords:Alkaline single cell gel electrophoresis Lymphocytes Radiation dose estimation |
| FundProject:国家自然科学基金资助项目(30570551);北京市自然科学基金资助项目(7053073) |
| Author Name | Affiliation | E-mail | | LIU Qing-jie | National Institute for Radiological Protection, China CDC, Beijing 100088, China | qingjieliu@yahoo.com | | LU Xue | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | FENG Jiang-bin | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | 陈德清 | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | 陈晓穗 | 海军总医院 | |
|
| Hits: 2733 |
| Download times: 1691 |
| Abstract:: |
| Objective To explore the feasibility of alkaline single cell gel electrophoresis (SCGE) in biological dosimetry of ionizing radiation. Methods Normal peripheral blood samples from two healthy males were exposed to different doses coblat-60 gamma-rays, ranged from 0 to 5 Gy, and the tail length (TL) and Oliver tail moment (TM) of the lymphocytes were analyzed with SCGE. The dose-effect curves of TL and TM were fitted respectively. The TL and TM of lymphocytes for eight radiation workers were analyzed with SCGE, cumulative doses were estimated using the fitted TL and TM equations, and then compared with the recorded monitoring doses. Results The TLs or TMs of normal human lymphocytes were increased with the irradiation doses, and its relationship can be fitted with a linear-quadratic equations: Y=13.59+20.87X-2.27X2 for TL, and Y=8.50+15.04X-1.43X2 for TM, respectively (Y denotes TL or TM value, X is radiation dose). The doses estimated with TM equation were closer to the recorded monitoring doses than that with TL equation. Conclusions The TM in lymphocytes analyzed with SCGE is a promising radiation biological dosimeter. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
