| HAN Guang,ZHOU Yun-feng,PENG Min.Establishment of mice model of radiation-induced lung injury[J].Chinese Journal of Radiological Medicine and Protection,2006,26(5):442-445 |
| Establishment of mice model of radiation-induced lung injury |
| Received:November 23, 2005 |
| DOI： |
| KeyWords:Mice Radiation-induced lung injury Mice model Hydroxyproline |
| FundProject:国家自然科学基金资助项目(30472263) |
| Author Name | Affiliation | E-mail | | HAN Guang | Department of Radio-chemotherapy, Zhongnan Hospital and Cancer Research Center, Wuhan University, Wuhan 430071, China | yunfeng_zhou@hotmail.com | | ZHOU Yun-feng | Department of Radio-chemotherapy, Zhongnan Hospital and Cancer Research Center, Wuhan University, Wuhan 430071, China | | | PENG Min | 武汉大学医学院病毒研究所 | | | 张明生 | Department of Radio-chemotherapy, Zhongnan Hospital and Cancer Research Center, Wuhan University, Wuhan 430071, China | | | 曹振 | Department of Radio-chemotherapy, Zhongnan Hospital and Cancer Research Center, Wuhan University, Wuhan 430071, China | | | 谢丛华 | Department of Radio-chemotherapy, Zhongnan Hospital and Cancer Research Center, Wuhan University, Wuhan 430071, China | | | 周福祥 | Department of Radio-chemotherapy, Zhongnan Hospital and Cancer Research Center, Wuhan University, Wuhan 430071, China | | | 张文杰 | Department of Radio-chemotherapy, Zhongnan Hospital and Cancer Research Center, Wuhan University, Wuhan 430071, China | |
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| Abstract:: |
| Objective To establish a mice model of radiation-induced lung injury. Methods Ninety C57BL/6 mice were randomly classified into two groups, one received sham-irradiation (control group, Group C, n=36) and another irradiated thoraces with single fraction 12-gray X-rays (treatment group, Group X, n=54). The mice were sacrificed at 1, 24, 72 hours and 1, 2, 4, 8, 16, 24 weeks after the irradiation, lung tissues were extracted and processed for various analyses including HE and Masson staining, and immunohistochemistry. Hydroxyproline contents were also measured. Results Compared with Group C, significant histological and pathologic changes were observed and confirmed with immunohistochemistry in Group X. Low level of hydroxyproline (0.763±0.029) μg/mg was recorded in Group C, and an elevated level of hydroxyproline (0.875±0.009) μg/mg was found in Group X. The difference between the two groups were statistically significant (P<0.05). Conclusions A mice model of radiation-induced lung injury was established successfully. |
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