| WANG Rui-juan,PENG Rui-yun,FU Kai-fei.Effect of rhIL-11 on expressions of interleukin-11 receptor α-chain and gp130 in IEC-6 cells after neutron irradiation[J].Chinese Journal of Radiological Medicine and Protection,2006,26(5):438-441 |
| Effect of rhIL-11 on expressions of interleukin-11 receptor α-chain and gp130 in IEC-6 cells after neutron irradiation |
| Received:October 13, 2005 |
| DOI： |
| KeyWords:Neutron IEC-6 Interleukin-11 IL-11Rα gp130 |
| FundProject:国家自然科学基金资助项目(30370438) |
| Author Name | Affiliation | E-mail | | WANG Rui-juan | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | pengry@nic.bmi.ac.cn | | PENG Rui-yun | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | FU Kai-fei | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 高亚兵 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 韩瑞刚 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 罗庆良 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 胡文华 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 马俊杰 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | |
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| Abstract:: |
| Objective To observe the effect of recombinant human interleukin-11(rhIL-11) on expressions of interleukin-11 receptor α-chain (IL-11Rα) and glycoprotein 130 (gp130), an additional signal transducer, in IEC-6 cells after neutron irradiation. Methods IEC-6 cells were exposed to 4.0 Gy neutron and administered with 100 ng/ml rhIL-11 12 h before or immediately after irradiation. The apoptosis and necrosis rates and expressions of IL-11Rα and gp130 were detected by flow cytometry, immunohistochemistry, Western blot, and image analysis. Results The apoptosis rate of IEC-6 cells was increased by irradiation at 6 h (P<0.01), IL-11 stimulation resulted in a decreased apoptosis rate in irradiated IEC-6 cells (P<0.05). In normal control IEC-6 cells, IL-11Rα was strongly expressed in the cellular membrane and cytoplasm. The level of IL-11Rα expression significantly decreased at 6 h after irradiation (P<0.01) and returned to the normal level at 24 h after the irradiation. In IEC-6 cells treated with both radiation and rhIL-11, the level of IL-11Rα expression was higher than that of the irradiated cells (P<0.05), while gp130 protein was strongly expressed in the cytoplasm of IEC-6 cells. After irradiation, we found a progressive decrease in the expression of gp130 protein (P<0.05) in 48 h, while in rhIL-11-stimulated cells, it returned to the normal level at 24 h after irradiation and decreased at 48 h, but still higher than that of the irradiated-only cells (P<0.05). Conclusions rhIL-11 can protect IEC-6 cells from neutron irradiation damage. The protection effect of rhIL-11 might be connected with its ability of up-regulating expressions of specific ligand-binding subunit IL-11Rα and signal-transducing subunit gp130. |
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