| ZHOU Yong,MI Man-tian,YANG Zhen-zhou.Effect of genistein on cell cycle of bone marrow hematopoietic cells in normal and irradiated mice[J].Chinese Journal of Radiological Medicine and Protection,2006,26(5):434-437 |
| Effect of genistein on cell cycle of bone marrow hematopoietic cells in normal and irradiated mice |
| Received:December 28, 2005 |
| DOI： |
| KeyWords:Genistein Bone marrow hematopoietic cells Cell cycle bcl-2 |
| FundProject:国家自然科学基金资助项目(30100046) |
| Author Name | Affiliation | | ZHOU Yong | Department of Nutrition and Food Hygiene, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | MI Man-tian | Department of Nutrition and Food Hygiene, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | YANG Zhen-zhou | 大坪医院野战外科研究所肿瘤中心 | | 朱俊东 | Department of Nutrition and Food Hygiene, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | 许红霞 | Department of Nutrition and Food Hygiene, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China |
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| Abstract:: |
| Objective To study the effects of genistein on cell cycle, proliferation and expression of bcl-2 gene in bone marrow hematopoietic cells (BMHCs) of normal and irradiated mice in order to explore mechanisms for protection of genistein from radiation-induced hematopoietic system injury. Methods Adult male BALB/c mice were orally administered with genistein (160 mg/kg b.w.) 24 h before irradiation. Cell cycles in BMHCs of the normal and irradiated mice were measured by flow cytometry. The protein and mRNA expressions of bcl-2 gene in BMHCs were analyzed by Western blot and RT-PCR, respectively. Results a) Transitory and significant changes occurred in the cell cycle of BMHCs in the normal mice after administration of genistein: first, the proliferation suppression of BMHCs was observed and most cells were arrested in G0/G1 phase on day 1; second, progression of cells from G0/G1 phase into S phase was observed, accumulation of cells in S phase on day 2, and back to the normal level on day 4. b) Genistein, administration 24 h before irradiation, decreased the percentage of BMHCs in G0/G1 phase and increased cell proliferation. Moreover, genistein up-regulated the protein and mRNA expressions of bcl-2 in BMHCs in the irradiated mice. Conclusions It was shown that changing with cell cycle, strengthening of radioresistant, suppressing of radiation-induced apoptosis, and enhancing of proliferation and differentiation of BMHCs maybe the underlying mechanisms for genistein protection of hematopoietic system against radiation damage. |
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