| WANG Zhuan-zi,LI Wen-jian,WANG Ju-fang.The linear correlation between human hepatoma cellular radiosensitivity and G2-chromosome aberration measured by the G2-PCC assay[J].Chinese Journal of Radiological Medicine and Protection,2006,26(5):431-433 |
| The linear correlation between human hepatoma cellular radiosensitivity and G2-chromosome aberration measured by the G2-PCC assay |
| Received:October 11, 2005 |
| DOI： |
| KeyWords:Premature chromosome condensation technique Cell survival fraction G2 iso- chromosome breakage G2 chromatid-type breakage Linear correlation |
| FundProject:国家自然科学基金重点资助项目(10335050);国家科技部重大基础研究前期研究专项(2003CCB00200) |
| Author Name | Affiliation | | WANG Zhuan-zi | Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China | | LI Wen-jian | Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China | | WANG Ju-fang | Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China | | 周清明 | Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China | | 谢漪 | Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China | | 毕先 | Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China | | 马爽 | Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China |
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| Abstract:: |
| Objective To investigate the correlation between G2-chromsome aberrations induced by γ-rays and intrinsic radiosensitivity and to see whether the result can be used to predict the cellular radiosensitivity. Methods HepG2 cells were irradiated with a range of doses and assessed both for cell survival and for residual G2-chromsome aberrations at 24 h after irradiation. The cell survival fraction was described by colony assay; the residual G2-chromsome aberrations including iso-chromatid breaks and chromatid-type breaks were detected by Calyculin-A induced premature chromosome condensation technique at 24 h after irradiation. Results The cell survival fraction decreased with the dose in linearly quadratic manner; the residual G2 iso-chromatid breaks per cell and G2 chromatid-type breaks per cell after 24 h of post-irradiation linearly increased with the dose. There is an inverse relationship between intrinsic radiosensitivity and G2 iso-chromatid breaks per cell (-0.989) or G2 chromatid-type breaks per cell (-0.853). Conclusions The residual G2 iso-chromatid breaks after 24 h of post-irradiation can represent the cell intrinsic radiosensitivity. |
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