| LIU Xiao-dong,LIU Yang,ZHAO Yin-long.Effects of ionizing radiation on morphological characteristics of prostate cells under 3-dimensional condition[J].Chinese Journal of Radiological Medicine and Protection,2006,26(5):427-430 |
| Effects of ionizing radiation on morphological characteristics of prostate cells under 3-dimensional condition |
| Received:April 15, 2006 |
| DOI： |
| KeyWords:Ionizing radiation 3-dimensional culture RWPE-1 Acini |
| FundProject:国家自然科学基金资助项目(30500142);教育部留学回国人员科研启动基金(2005年);吉林省科技厅基金(2005136);吉林大学创新基金资助项目(2004年) |
| Author Name | Affiliation | E-mail | | LIU Xiao-dong | School of Public Health, Jilin University, Changchun 130021, China | liuxd2002@yahoo.com | | LIU Yang | School of Public Health, Jilin University, Changchun 130021, China | | | ZHAO Yin-long | School of Public Health, Jilin University, Changchun 130021, China | | | 陈大伟 | 130021 长春, 吉林大学公共卫生学院卫生部放射防护教研室 | |
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| Abstract:: |
| Objective To observe the formation of acini structure of prostate cells under 3-dimensional (3_D) condition and the effects of radiation on it. Methods RWPE-1 cells were cultured under 3-D condition, Western blot was used to detect the expressions of Beclin 1,p53 and p21,in Beclin 1 knock-down cell model,i.e.,RWPE-Bi, immunofluorescence staining and confocal microscopy were used to detect the morphological characteristics of RWPE cells irradiated by 60Co. Results In 3_D culture,acini was observed,characterized by sphroid structure and cell polarity.After radiation,the acini was destructed and cell death was found,especially in RWPE-Bi compared with RWPE-1. Conclusions Radiation could suppress the formation of acini structure of prostate cells and Beclin 1 might play a role in this process. |
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