| YAO Xiao-wu,CHEN Shi-sheng,YANG Li-he.The effect of ionizing radiation on the filamentous actin of vascular endothelial cell[J].Chinese Journal of Radiological Medicine and Protection,2006,26(4):363-366 |
| The effect of ionizing radiation on the filamentous actin of vascular endothelial cell |
| Received:January 08, 2006 |
| DOI： |
| KeyWords:Ionizing radiation Vascular endothelial cell Filamentous actin |
| FundProject:广东省医学科研基金资助项目(A2003524) |
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| Abstract:: |
| Objective To observe the ionizing radiation effect on filamentous actin of vascular endothelial cell and explore its mechanism. Methods The vascular endothelial cells were irradiated with 0, 2, 4, 6, 8, 10 and 12 Gy 60Coγ -rays. The cytoskeleton was observed with CLSM at 6 hs after the irradiation and the cytoskeleton protein F-actin detected with flow cytometry after 12 and 24 hs. Results The damage to cytoskeletons increased with the radiation dose. The cytoskeleton protein F-actin was significantly decreased at 12 hs after the irradiation, and then recovered after 24 hs. Conclusion Ionizing radiation caused vascular endothelial cell injury by damaging the cytoskeleton and depolymerizating the F-actin. |
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