| XUE Xing-huan,MA Hong-Bing,WANG Xi-jing.Effects of p16 gene on the growth and radiation sensitivity of human pancreatic carcinoma cell line[J].Chinese Journal of Radiological Medicine and Protection,2006,26(4):343-345 |
| Effects of p16 gene on the growth and radiation sensitivity of human pancreatic carcinoma cell line |
| Received:April 24, 2006 |
| DOI： |
| KeyWords:Pancreatic carcinoma p16 gene Radiation sensitivity |
| FundProject:陕西省科技攻关基金资助项目(2002K10-G3);西安交通大学第二医院科研基金资助项目(2001YJ-04) |
| Author Name | Affiliation | E-mail | | XUE Xing-huan | Department of Oncology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China | mhbmali@yahoo.com.cn | | MA Hong-Bing | Department of Oncology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China | | | WANG Xi-jing | Department of Oncology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China | | | 狄政莉 | Department of Oncology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China | | | 刘小旭 | 西安市中心医院内科 | | | 马洁 | 中国医学科学院肿瘤研究所分子肿瘤学国家重点实验室 | | | 夏辉 | 中国医学科学院肿瘤研究所分子肿瘤学国家重点实验室 | | | 李铮 | 中国医学科学院肿瘤研究所分子肿瘤学国家重点实验室 | | | 韩志楷 | 中国医学科学院肿瘤研究所分子肿瘤学国家重点实验室 | | | 白明华 | Department of Oncology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China | |
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| Abstract:: |
| Objective To observe the effects of p16 gene transfection on the growth and radiation sensitivity of human pancreatic carcinoma cell lines. Methods Wild-type p16 cDNA was ligated to pcDNA3.1+ to construct pcDNA3.1+-p16 plasmid. Wild-type p16 gene was transfected into JF305 pancreatic carcinoma cell lines by lipofectamine TM, then the clones were randomly selected. Being irradiated,expression of exogenous p16 gene were detected with RT-PCR and Western blot. MTT assay was used to determine the curve of cell growth and the rate of damaged cells by irradiation. Results The integration and expression of p16 gene was demonstrated in JF305 cell after p16 transfected.The cell growth velocity was found obviously decreased and radiation sensitivity increased. Conclusion The transftection of p16 gene can inhibit malignant generation and increase sensitivity of human pancreatic carcinoma cells to radiation. |
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