| FENG Shuang,CAO Jian-ping,ZHU Wei.Radiation-induced effects of ATM gene on expressions of BRCA1 and RAD51 detected by laser scanning confocal microscopy[J].Chinese Journal of Radiological Medicine and Protection,2006,26(4):334-339 |
| Radiation-induced effects of ATM gene on expressions of BRCA1 and RAD51 detected by laser scanning confocal microscopy |
| Received:August 22, 2005 |
| DOI： |
| KeyWords:ATM gene BRCA1 RAD51 Radiation Laser scanning confocal microscopy |
| FundProject:国家自然科学基金资助项目(30170288);江苏省高校自然科学基金资助项目(04KJA180121);苏州大学江苏省级重点实验室开放经费资助项目(KJS05029);江苏省高校基金资助项目(05KJA33013) |
| Author Name | Affiliation | E-mail | | FENG Shuang | School of Radiology &
Public Health, Soo Chow University, Suzhou 215123, China | jpcao@suda.edu.cn | | CAO Jian-ping | School of Radiology &
Public Health, Soo Chow University, Suzhou 215123, China | | | ZHU Wei | School of Radiology &
Public Health, Soo Chow University, Suzhou 215123, China | | | 李冰燕 | School of Radiology &
Public Health, Soo Chow University, Suzhou 215123, China | | | 罗加林 | School of Radiology &
Public Health, Soo Chow University, Suzhou 215123, China | | | 盛方军 | School of Radiology &
Public Health, Soo Chow University, Suzhou 215123, China | | | 周新文 | School of Radiology &
Public Health, Soo Chow University, Suzhou 215123, China | | | 宋建元 | School of Radiology &
Public Health, Soo Chow University, Suzhou 215123, China | | | 李翀 | School of Radiology &
Public Health, Soo Chow University, Suzhou 215123, China | | | 樊赛军 | School of Radiology &
Public Health, Soo Chow University, Suzhou 215123, China | |
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| Abstract:: |
| Objective To investigate the expressions of BRCA1 and RAD51 proteins in GM, ATM+-AT, and AT cells before and after irradiation by 60Coγ -rays. Methods The localizations and expressions of BRCA1 and RAD51 proteins in GM, ATM+-AT, AT cells were detected by immunofluorescence staining and laser scanning confocal microscopy after 0 and 10 Gy 60Coγ -ray irradiation. Quantitative analysis was also processed on these proteins. Results Before irradiation with γ-rays, few expression of RAD51 or BRCA1 protein and no colocalization was observed in GM, ATM+-AT, AT cells, and lowest expression in AT cells. Being irradiated with 10 Gy 60Coγ -rays, the expressions of BRCA1 and RAD51 proteins were increased in GM, ATM+-AT, and AT cells. Colocalized expressions in GM, ATM+-AT cells were observed, but no such expression in AT cells. The expressions of BRCA1 and RAD51 proteins in GM, ATM+-AT cells statistically significantly differed from those in AT cells (P<0.01). The difference of the expression of BRCA1 and RAD51 proteins between GM cells and ATM+-AT cells was also statistically significant (P<0.01). Conclusions After induction by irradiation, ATM gene that exists in GM and ATM+-AT cells could activate its down stream genes, BRCA1 and RAD51, and could enhance the expressions of and interactions between these two proteins. All these changes are helpful for cell repair following radiation injury. The expressions of BRCA1 and RAD51 proteins were lower in normal GM cells transfected with extrinsic ATM than that in GM cells because the extrinsic ATM gene could not completely compensate the function, partial phosphorylation of down stream gene BRCA1, of intrinsic ATM mutative gene. |
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