| ZHOU Zhou,ZHANG Lei,YU Xiao-dong.Radiation-induced activation of GSK-3 β-coupled signal transduction pathway in IEC-6 cells[J].Chinese Journal of Radiological Medicine and Protection,2006,26(4):328-331 |
| Radiation-induced activation of GSK-3 β-coupled signal transduction pathway in IEC-6 cells |
| Received:July 08, 2005 |
| DOI： |
| KeyWords:γ-ray GSK-3 β caspase-9 caspase-3 |
| FundProject:国家自然科学基金资助项目(30300072) |
| Author Name | Affiliation | E-mail | | ZHOU Zhou | Department of Occupational Health, Third Military Medical University, Chongqing 400038, China | yuzping@yahoo.com | | ZHANG Lei | Department of Occupational Health, Third Military Medical University, Chongqing 400038, China | | | YU Xiao-dong | Department of Occupational Health, Third Military Medical University, Chongqing 400038, China | | | 邓朝晖 | Department of Occupational Health, Third Military Medical University, Chongqing 400038, China | | | 张广斌 | Department of Occupational Health, Third Military Medical University, Chongqing 400038, China | | | 余争平 | Department of Occupational Health, Third Military Medical University, Chongqing 400038, China | |
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| Abstract:: |
| Objective To determine the effects of γ radiation on GSK-3 β-coupled signaling transduction pathway in IEC-6 cells. Methods The changes of GSK-3 β, Akt, caspase-9, and caspase-3 mRNA expressions in IEC-6 cells after 6 Gy γ-ray irradiation were determined using RT-PCR. GSK-3 β and Akt phosphorylation levels were determined by Western blot. Changes of caspase-9 enzymes activity were measured by colorimetric assay. Results GSK-3 β mRNA expressions at 30, 60 and 120 min after exposure were observed to be increased with 6 Gy γ-ray irradiation, while the corresponding levels of phosphorylated form of GSK-3 β decreased. Akt mRNA expression in the exposed group was not significantly different from that in the control group, but phosphorylation of Akt decreased at 30, 60, 120 min after exposure to 6 Gy γ-ray. Caspase-9 mRNA expression increased at 30, 60, 120 min and caspase-3 mRNA expression increased at 60, 120 min after the exposure. In consistence with the expression of caspase-9 mRNA, caspase-9 activity was markedly elevated at 30, 60, 120 min after exposure. Conclusion γ-ray irradiation induced significant activations of GSK-3 β signal transduction pathway and apoptosis-related caspase-9 and caspase-3 enzymes in IEC-6 cells. |
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