| TANG Gu-Sheng,CAI Jian-Ming,NI Jin.Study on radiosensitivity of B16 cells transfected by STAT3 antisense oligodeoxynucleotides[J].Chinese Journal of Radiological Medicine and Protection,2006,26(4):324-327 |
| Study on radiosensitivity of B16 cells transfected by STAT3 antisense oligodeoxynucleotides |
| Received:January 08, 2006 |
| DOI： |
| KeyWords:ASO-STAT3 B16 Radiosensitivity Apoptosis Tumor therapy |
| FundProject:国家自然科学基金资助项目(30470415) |
| Author Name | Affiliation | E-mail | | TANG Gu-Sheng | Department of Radiation and Nuclear Medicine, The Second Military Medical University, Shanghai 200433, China | cjm882003@yahoo.com.cn | | CAI Jian-Ming | Department of Radiation and Nuclear Medicine, The Second Military Medical University, Shanghai 200433, China | | | NI Jin | Department of Radiation and Nuclear Medicine, The Second Military Medical University, Shanghai 200433, China | | | 吴君 | 第二医科大学附属仁济医院急诊科 | | | 项莺松 | Department of Radiation and Nuclear Medicine, The Second Military Medical University, Shanghai 200433, China | | | 崔建国 | Department of Radiation and Nuclear Medicine, The Second Military Medical University, Shanghai 200433, China | | | 单鹄声 | Department of Radiation and Nuclear Medicine, The Second Military Medical University, Shanghai 200433, China | | | 赵芳 | Department of Radiation and Nuclear Medicine, The Second Military Medical University, Shanghai 200433, China | |
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| Abstract:: |
| Objective To investigate the influence of STAT3 antisense oligodeoxynucleotides (ASO-STAT3) on radiosensitivity of B16 cells. Methods Oligodeoxynucleotides were tansfected into B16 cells with oligofectamine reagent. Cell survival was measured using CCK-8 assay. Apoptotic assays were performed using Hoechst33258 staining and Annexin V/PI with FACS analysis. Results Cell survival decreased significantly in those groups tansfected with ASO-STAT3 and irradiated with different dose of γ-rays. Compared with those cells treated with irradiation alone or AO-STAT3 transfection with subsequent exposure to radiation, fraction of early apoptosis increased in those cells treated with ASO-STAT3 transfection combined with irradiation. Conclusion These results showed that targeting ASO-STAT3 can enhance the therapeutic effect of γ-ray irradiation on B16 cells, which implied that STAT3 can act as a potential molecular target for tumor therapies, and also a potential molecular target for enhancement of radiosensitivity of radioresistant tumors. |
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