| XIANG Qing,XU Mei,XIAO Cheng.Changes of tumorigenicity and gene expressions of melanoma cells mutated in outer space[J].Chinese Journal of Radiological Medicine and Protection,2006,26(4):320-323 |
| Changes of tumorigenicity and gene expressions of melanoma cells mutated in outer space |
| Received:November 07, 2005 |
| DOI： |
| KeyWords:Melanoma B16 cell line Gene expression Outer space |
| FundProject:国家重点基础研究规划“973”计划前期资助项目(2003CA04200);国家自然科学基金资助项目(30371324) |
| Author Name | Affiliation | E-mail | | XIANG Qing | Clinical Medical Science Institute, China-Japan Friendship Hospital, Beijing 100029, China | tangjt@mail.tsinghua.edu.cn | | XU Mei | Clinical Medical Science Institute, China-Japan Friendship Hospital, Beijing 100029, China | | | XIAO Cheng | Clinical Medical Science Institute, China-Japan Friendship Hospital, Beijing 100029, China | | | 徐波 | Clinical Medical Science Institute, China-Japan Friendship Hospital, Beijing 100029, China | | | 李红艳 | Clinical Medical Science Institute, China-Japan Friendship Hospital, Beijing 100029, China | | | 郭宇鹏 | 昆明医学院第一附属医院胸外科 | | | 耿传营 | Clinical Medical Science Institute, China-Japan Friendship Hospital, Beijing 100029, China | | | 潘琳 | Clinical Medical Science Institute, China-Japan Friendship Hospital, Beijing 100029, China | | | 房青 | Clinical Medical Science Institute, China-Japan Friendship Hospital, Beijing 100029, China | | | 唐劲天 | 清华大学医学物理与工程研究所 | |
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| Abstract:: |
| Objective To screen the cell lines with decreased tumorigenicity, and identify those genes related to the development and metastasis of melanoma. Methods The murine melanoma B16 cells were carried into the outer space by No.20 retrievable satellite, and the survival cells were cloned after returned to earth. Five monoclonal cell lines(1#,5#,6#,7#,8#)were utilized for further study. The cells were injected into C57BL/6J mice subcutaneously and abdominally respectively, then tumor-free time and survival time were recorded, tumor lumps were examined by routine pathological method. Gene chips were used to detect the gene expressions in 2 cell lines(1#,8#)with decreased tumorigenicity. Results Compared with the control group, the tumor-free time was longer for 1# cell lines (P<0.05). The survival time was significantly increased (P<0.01)and the weights of tumors were significantly decreased (P<0.01)for both 1# and 8# cell lines. The lymphocytes were infiltrated into tumors and adjacent tissues in those mice injected with 1# and 8# cell lines. Changes in 145 gene expressions were identified in 1# cell lines, and 124 genes in 8# cell lines (P<0.05), 9 genes of them were common to both cell lines. Furthermore, prostaglandin D2 synthase gene was markedly upregulated. Conclusion The study implied that the decrease of tumorigenicity was related to the changes of carcinoma-associated gene expressions. |
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