| WANG Feng-chao,RAN Xin-ze,DU Zhi-yong.Ionizing radiation can influence the expression of NF-κB p65 and Akt in the intestinal epithelial cell[J].Chinese Journal of Radiological Medicine and Protection,2006,26(4):317-319 |
| Ionizing radiation can influence the expression of NF-κB p65 and Akt in the intestinal epithelial cell |
| Received:December 24, 2005 |
| DOI： |
| KeyWords:IEC-6 PI3K/Akt NF-κB p65 Irradiation |
| FundProject:国家自然科学基金重点资助项目(30230360);国家自然科学基金青年基金资助项目(30400247) |
| Author Name | Affiliation | E-mail | | WANG Feng-chao | State Key Laboratory of Trama, Burn and Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | suyp2003@yahoo.com.cn | | RAN Xin-ze | State Key Laboratory of Trama, Burn and Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | DU Zhi-yong | State Key Laboratory of Trama, Burn and Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | 艾国平 | State Key Laboratory of Trama, Burn and Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | 王军平 | State Key Laboratory of Trama, Burn and Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | 粟永萍 | State Key Laboratory of Trama, Burn and Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | |
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| Abstract:: |
| Objective To explore the activation of PI3K/Akt pathway and NF-κB pathway in the intestinal epithelial cell stimulated by ionizing radiation. Methods The total cell protein, plasmatic and nuclear protein of normal IEC-6 cells and cells at 15 min, 1 h, 4 h after exposed to 12 Gy 60Coγ radiation were extracted and the expression levels of phosphorylated Akt(Ser 473) and the NF-κB p65 were tested by western blot. Results The translocation of p65 occurred 20 minutes after irradiation,but the obvious phosphorylation of Akt could not be found until 1 h after irradiation. Conclusion The nuclear translocation of NF-κB in the intestinal cell exposed to ionizing radiation is earlier than the activation of PI3K/Akt pathway stimulated by the same agent. |
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