| ZONG Zhao-wen,CHENG Tian-min,RAN Xin-ze.Study on mechanisms of preferential distribution of the transplanted dermal multipotent stem cells in rats with combined radiation and wound injury[J].Chinese Journal of Radiological Medicine and Protection,2006,26(4):313-316 |
| Study on mechanisms of preferential distribution of the transplanted dermal multipotent stem cells in rats with combined radiation and wound injury |
| Received:January 08, 2006 |
| DOI： |
| KeyWords:Dermal multipotent stem cell Combined radiation and wound injury Distribution Stromal-dereived factor-1 |
| FundProject:国家重点基础研究发展规划(973计划)资助项目(2005CB522605);国家自然科学基金资助项目(30500141);第三军医大学科研基金资助项目(XG200509) |
| Author Name | Affiliation | E-mail | | ZONG Zhao-wen | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | suyongping@yahoo.com.cn | | CHENG Tian-min | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | RAN Xin-ze | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | 李楠 | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | 董世武 | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | 艾国平 | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | 粟永萍 | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | |
|
| Hits: 2847 |
| Download times: 1772 |
| Abstract:: |
| Objective To explore the mechanisms of preferential distribution of transplanted dermal multipotent stem cells (dMSCs) to surface of wound in rats with combined radiation and wound injury (CRWI). Methods The expression of stromal-dereived factor-1 (SDF-1) in wounds of rats and the expression of CR4 (the only known receptor of SDF-1) in dMSCs were examined using RT-PCR and immunohistochemistry. The chemotactic effect of SDF-1 on dMSCs was evaluated in Transwell culture system. Results The expression of SDF-1 in wound of CRWI rats was upregulated significantly compared with the normal rats'skin. dMSCs expressed CR4. SDF-1 had a strong chemotactic effect on dMSCs; the chemotactic effect of wound extracts from CRWI rats was significantly reduced (P<0.05) when neutralized antibody to SDF-1 was added. Conclusion The upregulated expression of SDF-1 played an important role in the preferential distribution of transplanted dMSCs to wound in rats with CRWI. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
