| PAN Min-hong,PENG Rui-yun,GAO Ya-bing,et al.Expression of β1-adrenergic receptor in rat heart irradiated with high power microwave[J].Chinese Journal of Radiological Medicine and Protection,2006,26(3):298-300,287 |
| Expression of β1-adrenergic receptor in rat heart irradiated with high power microwave |
| Received:June 27, 2005 |
| DOI： |
| KeyWords:High power microwave Heart β_ 1 -adrenergic receptor Immunohistochemistry RT-PCR |
| FundProject: |
| Author Name | Affiliation | E-mail | | PAN Min-hong | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | pengry@nic.bmi.ac.cn | | PENG Rui-yun | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | GAO Ya-bing | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 王水明 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 马俊杰 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 王德文 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 胡文华 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 王晓民 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 苏镇涛 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | |
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| Abstract:: |
| Objective To investigate the effect of high power microwave(HPM) radiation on the expression of β1-adrenergic receptor(β1-AR) in rat heart. Methods S-band HPM device with mean power density 50 mW/cm2 was used to radiate 45 healthy Wistar male rats. Light microscope, immunohistochemistry, reverse transcriptase polymerase chain reaction(RT-PCR) and image analysis were used to study the pathological characteristics of heart tissue and the expression of β1-AR on the protein and mRNA level. Results 50 mW/cm2 HPM radiation caused progressive cardiomyofilaments derangement, degeneration and sarcoplasm condensation within 7 d. β1-AR protein was increased in endocardium and myometrium, and reached a peak on 3 d(P<0.05) and returned to the normal level on 14 d. Transcription of β1-AR mRNA was upregulated in endocardium and myometrium, and peaked peaked on 6 h-28 d (P<0.01); the mRNA level progressively incresed with time since irradiation except on 7 d. Conclusion 50 mW/cm2 HPM radiation injured heart tissue and increase the expression of β1-AR playing an important role in the pathophysiology of heart injury induced by HPM. |
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