| YAO Zhi_jun,CHEN Yong_bin,GUO Guo_zhen.Radioprotective effect of 5,10,15,20-tetrakis(4-hydroxyphenyl) porphyrin on endothelial cell of vein-304[J].Chinese Journal of Radiological Medicine and Protection,2006,26(3):295-297 |
| Radioprotective effect of 5,10,15,20-tetrakis(4-hydroxyphenyl) porphyrin on endothelial cell of vein-304 |
| Received:August 08, 2005 |
| DOI： |
| KeyWords:Electromagnetic pulse Porphyrin ECV cells |
| FundProject:国家自然科学基金资助项目(50372084) |
| Author Name | Affiliation | E-mail | | YAO Zhi_jun | Department of Chemistry, Fourth Military Medical University, Xi'an, 710032, China | lisydcn@yahoo.com.cn | | CHEN Yong_bin | 710003 西安, 第四军医大学预防医学系放射医学教研室 | | | GUO Guo_zhen | 710003 西安, 第四军医大学预防医学系放射医学教研室 | | | 孙晓莉 | Department of Chemistry, Fourth Military Medical University, Xi'an, 710032, China | |
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| Abstract:: |
| Objective To study the protective effect of 5,10,15,20-tetrakis (4hydroxyphenyl) porphyrin (THP) on endothelial cell of vein-304 (ECV-304) exposed to electromagnetic pulse (EMP). Methods MTT method was used to determine the optimal concentration of THP toxicity reaction for ECV-304 cells, and then used this concentration in the protection test. Chemiluminescence was used to measure the radioprotective effect of THP on ECV-304 cells exposed to varying intensity of EMP at 100,200, or 400 kV/m. ANOVA and independent samples t-test were used to analyze the data. Results The optimal concentration of THP for ECV-304 cells was estimated to be 0.01 μg/ml.The chemiluminescence intensities of ECV-304 cells administered with THP were statistically significantly lower than those in the control group. Conclusion This study suggested that 5,10,15,20-tetrakis (4hydroxyphenyl) porphyrin at 0.01 μg/ml can exert radioprotective effect of certain extent. |
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