| ZHANG Zun-zhen,ZHANG Qin,WU Mei.Effects of 60Co γ rays on radiosensitivity of down-regulated cell strain hOGG1 expressed genes[J].Chinese Journal of Radiological Medicine and Protection,2006,26(3):238-241 |
| Effects of 60Co γ rays on radiosensitivity of down-regulated cell strain hOGG1 expressed genes |
| Received:April 29, 2005 |
| DOI： |
| KeyWords:hOGG1 Radiosensitivity DNA damage and repair Cell cycle |
| FundProject:国家自然科学基金(30571535);高等学校博士学科点专项科研基金资助项目(20040610082) |
| Author Name | Affiliation | | ZHANG Zun-zhen | Department of Environmental Health, School of Public Health, Sichuan University, Sichuan, Chengdu 610041, China | | ZHANG Qin | Department of Environmental Health, School of Public Health, Sichuan University, Sichuan, Chengdu 610041, China | | WU Mei | Department of Environmental Health, School of Public Health, Sichuan University, Sichuan, Chengdu 610041, China | | 李娜 | Department of Environmental Health, School of Public Health, Sichuan University, Sichuan, Chengdu 610041, China | | 衡正昌 | Department of Environmental Health, School of Public Health, Sichuan University, Sichuan, Chengdu 610041, China |
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| Abstract:: |
| Objective To investigate the effect of 60Co γ rays on radiosensitivity of down-regulated cell strain hOGG1 expressed genes. Methods Human lung adenocarcinoma A549 cells and A549-R cells into which ribozyme genes (which inhibited the hOGG1 mRNA expression) were transfected were study materials. The cell survival rate after irradiation with different doses of 60Co γ rays was measured by MTT test. DNA damage and its repair were measured by single cell gel electrophoresis assay (SCGE). Flow cytometry was used to determine the apoptotic cell population, cell cycle distribution and cell proliferation index. Results The cell survival rate after irradiation was lower in the A549-R cells than that in the A549 cells. DNA damage induced by 2, 4, 8 or 12 Gy of 60Co γ rays was nearly the same in the two cell strains. The DNA repair showed a big difference in the two cell strains. The repair capability in the A549-R cells was significantly lower than that in the A549 cells. The percentages of A549-R cells in G0/G1 phase were increased with the gamma irradiation. Additionally, the percentages of cells in S and G2/M phase were significantly decreased in the two cell strains. The apoptosis percentages of the A549-R cells were obviously higher than those of the A549 cells when irradiated with the same dose of gamma rays. Both cell strains showed that the G0/G1 arrest, and cell proliferation index was decreased. Conclusion hOGG1 ribozyme increased the sensitivity to 60Co γ rays in the A549 cells. |
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