| DENG Jun,ZHOU Tao-li,ZHANG Chun-lei.Study on nitric oxide enhancement effect of radiation damage to L1210 cells[J].Chinese Journal of Radiological Medicine and Protection,2006,26(3):233-235 |
| Study on nitric oxide enhancement effect of radiation damage to L1210 cells |
| Received:June 29, 2005 |
| DOI： |
| KeyWords:NO Apoptosis L1210 |
| FundProject: |
| Author Name | Affiliation | | DENG Jun | Department of Clinical Hematology, Third Military Medical University, chongqing 400038, China | | ZHOU Tao-li | 基础部病原生物学教研室 | | ZHANG Chun-lei | Department of Clinical Hematology, Third Military Medical University, chongqing 400038, China | | 郑峻松 | Department of Clinical Hematology, Third Military Medical University, chongqing 400038, China | | 李招权 | Department of Clinical Hematology, Third Military Medical University, chongqing 400038, China | | 黄辉 | Department of Clinical Hematology, Third Military Medical University, chongqing 400038, China | | 陈晓莉 | Department of Clinical Hematology, Third Military Medical University, chongqing 400038, China | | 程平 | Department of Clinical Hematology, Third Military Medical University, chongqing 400038, China |
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| Abstract:: |
| Objective To study whether nitric oxide(NO) could enhance the damage effect of radiation on L1210 cells in vitro. Methods L1210 cells co-cultured with different transfected 3T3 cells. The cells were divided into three groups, iPT group, PT group, and iPT plus DEVD-CHO group. The direct effect of NO and NO-induced apoptosis of L1210 cells in different groups at 12,24,48 and 72 h were detected by trypan blue rejecting dyeing (TRD) and TUNNEL, respectively. The cell cycles were detected by flow cytometry. Results The rate of TRD of L1210 cells during the time period of 12-72 h since irradiation was significantly lower in iPT group than that in PT group (P< 0.05). There was also significant difference between iPT group and iPT plus DEVD-CHO group during the time period of 24-72 h since irradiation (P<0.05). The apoptosis of L1210 cells during the time period of 24-72 h since irradiation in iPT group was significantly higher than that in PT group (P<0.05). There was also significant difference between iPT group and iPT plus DEVD-CHO group during the time period of 24-72 h since irradiation (P<0.05). L1210 cells at G1 phase at 4 h since irradiation increased more quickly in iPT group but declined more slowly during the time period of 24-72 h since irradiation than that in PT group (P<0.05). In iPT plus DEVD-CHO group,L1210 cells at G1 phase increased more slowly at 4 h than that in iPT group but declined more quickly during the time period of 24-72 h since irradiation(P<0.05). Conclusion NO can enhance the damage of radiation to L1210 cells through direct damage to L1210 cells; enhancement of apoptosis and blockage of G1 phase are dependent on Caspase-3 activation. |
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