| LI Ping,YANG Zhi-hua,PAN Xiu-jie.Study on cellular genotoxicities induced by alpha particles irradiation in combination with NNK treatment[J].Chinese Journal of Radiological Medicine and Protection,2006,26(3):229-232 |
| Study on cellular genotoxicities induced by alpha particles irradiation in combination with NNK treatment |
| Received:January 10, 2006 |
| DOI： |
| KeyWords:Alpha particles irradiation NNK BEP2D cells DNA damage HPRT gene mutation Micronucleus |
| FundProject: |
| Author Name | Affiliation | E-mail | | LI Ping | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | zhumx@nic.bmi.ac.cn | | YANG Zhi-hua | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | PAN Xiu-jie | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 曹珍山 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 米娜 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 陈忠民 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 刘刚 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 魏菡 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 李慧颖 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | 朱茂祥 | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | |
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| Abstract:: |
| Objective To investigate cellular genotoxicities of aplha particles irradiation in combination with NNK treatment. Methods Exponentially growing immortalized human bronchial epithelial cells were divided into the normal control group (NC), alpha particles irradiation (α),NNK administration group (NNK), NNK administration (100 μg/ml) followed by alpha particles irradiation group (NNK+α), and alpha particles irradiation followed by NNK administration (100 μg/ml) group (α+NNK). DNA damage were detected by single cell gel electrophoresis(SCGE); multinuclear cell assay was used to detect the frequency of the HPRT gene mutation; cell micronucleus frequency were detected by cytogenetic methods. Results In the group exposed to both alpha particles irradiation and NNK, DNA damage, HPRT gene mutation frequency, and cell micronucleus frequency were significantly higher than those in the same dose groups irradiated with alpha particles or NNK administration alone. Subtracted the NNK effect, DNA damage, HPRT gene mutation frequency and cell micronucleus frequency in the group irradiated by alpha particles in combination with NNK administration were significantly higher than those of alpha particles irradiation alone. Conclusion The genotoxicity of alpha particles irradiation in combination with NNK administration had synergistic effect. |
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