| LIU Jian-xiang,RUAN Jian-lei,SU Xu,et al.Overexpression of TNFα gene in SMMC-7721 cells irradiated with 12C6+ ion[J].Chinese Journal of Radiological Medicine and Protection,2006,26(3):224-226 |
| Overexpression of TNFα gene in SMMC-7721 cells irradiated with 12C6+ ion |
| Received:June 27, 2005 |
| DOI： |
| KeyWords:Carbon ion Human liver cancer cell line SMMC-7721 TNFα expression |
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| Abstract:: |
| Objective To investigate the expression of pEgr-TNFα in SMMC-7721 cells, which were transfected by polyfect transfection reagent and irradiated with carbon ion 12C6+ ion. Methods The protein expression of TNFα was measured with ELISA, its transcription level was determined by RT-PCR. Results The expression of TNFα was measured to be 2.06 ng/ml when transfected by pEgr-TNFα, and increased to 2.11 ng/ml with irradiation of 2 Gy 12C6+, which was significantly higher than that of the control group or the vector group. The transcription level of TNFα was increased with the transfection; more significant higher level was observed with irradiation of 2 Gy 12C6+. Conclusion Carbon ions combined with pEgr-TNFα can significantly increase TNFα expression level in SMMC-7721 cells. |
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