| GENG Chuan-ying,XIANG Qing,XU Mei.Effects of space environment on biological characteristics of melanoma B16 cells[J].Chinese Journal of Radiological Medicine and Protection,2006,26(3):207-209 |
| Effects of space environment on biological characteristics of melanoma B16 cells |
| Received:November 07, 2005 |
| DOI： |
| KeyWords:Space environments Melanoma B16 Mutagenesis Differentiation |
| FundProject:国家自然科学基金资助项目(30371324);国家科技部“973”前期资助项目[2003(CA04200)] |
| Author Name | Affiliation | E-mail | | GENG Chuan-ying | Department of Biochemistry and Molecular Biology, China-Japan Friendship Institute of Clinical Medical Sciences, Beijing 100029, China | tjt@263.net | | XIANG Qing | Department of Biochemistry and Molecular Biology, China-Japan Friendship Institute of Clinical Medical Sciences, Beijing 100029, China | | | XU Mei | Department of Biochemistry and Molecular Biology, China-Japan Friendship Institute of Clinical Medical Sciences, Beijing 100029, China | | | 郭宇鹏 | 昆明医学院第一附属医院胸外科 | | | 李红艳 | Department of Biochemistry and Molecular Biology, China-Japan Friendship Institute of Clinical Medical Sciences, Beijing 100029, China | | | 徐波 | Department of Biochemistry and Molecular Biology, China-Japan Friendship Institute of Clinical Medical Sciences, Beijing 100029, China | | | 房青 | Department of Biochemistry and Molecular Biology, China-Japan Friendship Institute of Clinical Medical Sciences, Beijing 100029, China | | | 唐劲天 | 清华大学工程物理系医学物理与工程研究所 | |
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| Abstract:: |
| Objective To examine the effects of space environment on biological characteristics of melanoma B16 Cells. Methods B16 cells were carried to the space (in orbit for 8 days, circle the earth 286 times) by the 20th Chinese recoverable satellite,and then harvested and monocloned.110 strains of space B16 cells were obtained in total.Ten strains of space B16 cells were selected and its morphological changes were examined with the phasecontrast microscope.Flow cytometry and MTT assay were carried out to evaluate the cell cycle and cell viability. Results Morphological changes were observed in the space cells, and melainin granules on the surface in some cells. It was demonstrated by MTT assay that space cells viability varied muti-directionally. It was showed by flow cytometry analysis that G1 phase of space cells was prolonged, S phase shortened. Conclusion Space environment may change the biological characteristics of melanoma B16 cells. |
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