| WANG Hui-ping,XU Qin-zhi,HUANG Yue_cheng.Effects of siRNA-silenced CHD6 on radiosensitivity in A549 cells[J].Chinese Journal of Radiological Medicine and Protection,2006,26(3):203-206 |
| Effects of siRNA-silenced CHD6 on radiosensitivity in A549 cells |
| Received:August 22, 2005 |
| DOI： |
| KeyWords:Chromatin reconstitution siRNA Cell cycle Cell proliferation Radiosensitivity |
| FundProject:国家自然科学基金资助项目(30270423);国家“863”计划基金资助项目(2004AA221160) |
| Author Name | Affiliation | E-mail | | WANG Hui-ping | Institute of Radiation Medicine, Beijing 100850, China | Zhoupk@nic.bmi.ac.cn | | XU Qin-zhi | Institute of Radiation Medicine, Beijing 100850, China | | | HUANG Yue_cheng | Institute of Radiation Medicine, Beijing 100850, China | | | 隋建丽 | Institute of Radiation Medicine, Beijing 100850, China | | | 白贝 | Institute of Radiation Medicine, Beijing 100850, China | | | 周平坤 | Institute of Radiation Medicine, Beijing 100850, China | |
|
| Hits: 2787 |
| Download times: 1918 |
| Abstract:: |
| Objective To observe the effects of siRNA-silenced chromatin reconstitution protein CHD6 on the proliferation and radiosensitivity in A549 cells. Methods CHD6-silenced cellular model was constructed by plasmid mediated siRNA technique.RT-PCR was used to detect the expression of mRNA.Growth curve,flow cytometry and fluorescent staining method were used to measure changes of cell proliferation,cell cycle and apoptosis,respectively.The radiosensitivity of A549 cells was determined with cell cloning efficiency test. Results siRNA-asilence CHD6 increased the growth of A549 cells and enhanced the radiation resistance of A549 cells when gamma irradiation dose was lesser than two grays, whereas it had no significant effect on apoptosis in cells being irradiated with larger doses. Conclusion Inhibition of CHD6 could enhance cell proliferation and cell radiosensitivity. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
