| SHI De-gang,SHI Gen-ming,HUANG Gang.Chemosensitivity of irradiated resistant cells of multicellular spheroids in A549 lung adenocarcinoma[J].Chinese Journal of Radiological Medicine and Protection,2006,26(2):155-158 |
| Chemosensitivity of irradiated resistant cells of multicellular spheroids in A549 lung adenocarcinoma |
| Received:August 10, 2005 |
| DOI： |
| KeyWords:Lung adenocarcinoma Multicellular spheroids Irradiation resistant Chemosensitivity |
| FundProject:国家自然科学基金资助项目(30470497) |
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| Abstract:: |
| Objective To investigate the chemosensitivity of irradiated resistant cells of multicellular spheroids in A549 lung adenocarcinoma. Methods The A549 irradiated resistant cells were the 10th regrowth generations after irradiated with 25 Gy of 6 MV X-ray, with the control groups were A549 parent cells and MCF7/VCR resistant cells. The 6 kinds of chemotherapeutic drugs were DDP,VDS,5-FU,HCP,MMC and ADM respectively, with verapamil (VPL) as reverse agent. The treatment effect was compared with MTT assay, and the multidrug resistant gene expressions of mdr1 and MRP were measured with RT-PCR method. Results A549 cells and irradiated resistant cells were resistant to DDP, but sensitivity to VDS,5-FU,HCP,MMC and ADM. The inhibitory rates of VPL to the above two cells were 98% and 25% respectively(P<0.001). The A value of the control A549 cells was 2 times of irradiated resistant cells (P<0.001).MCF7/VCR cells were resistant to DDP and MMC, but slight sensitivity to VDS,5-FU,HCP and ADM, with 80% of inhibitory rate to VPL. mdr1/β2-MG and MRP/β2-MG of all A549 cells were about 0 and 0.7 respectively, and those of MCF7/VCR cells were 35 and 4.36. Conclusion The chemosensitivity of A549 irradiated resistant cells had not changed markedly,the decreased sensitivity to VPL could not be explained by the gene expression of mdr1 and MRP. It is conferred that some kinds of changes in the cell membrane and decreased regrowth ability to result in resistance. Unlike multidrug resistance induced by chemotherapy, VPL may be not an ideal reverser to irradiated resistant cells. The new kinds of biological preparation should be seeked to combine chemotherapy to treat recurring tumor with irradiated resistance. |
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