| ZHANG Long-zhen,CAO Yuan-dong,TANG Tian-you,et al.Establishment of rat model of opening blood-brain barrier with conventional whole-brain irradiation[J].Chinese Journal of Radiological Medicine and Protection,2006,26(2):148-150 |
| Establishment of rat model of opening blood-brain barrier with conventional whole-brain irradiation |
| Received:June 29, 2005 |
| DOI： |
| KeyWords:Radiotherapy Animal model Blood-brain barrier |
| FundProject:江苏省卫生厅资助项目(H200534);江苏省教育厅自然科学基金资助项目(01KJD320033) |
| Author Name | Affiliation | | ZHANG Long-zhen | Department of Radiation Oncology, Xuzhou Medical College Cancer Institute, Xuzhou 221002, China | | CAO Yuan-dong | Department of Radiation Oncology, Xuzhou Medical College Cancer Institute, Xuzhou 221002, China | | TANG Tian-you | Department of Radiation Oncology, Xuzhou Medical College Cancer Institute, Xuzhou 221002, China | | 陈勇 | Department of Radiation Oncology, Xuzhou Medical College Cancer Institute, Xuzhou 221002, China | | 董红燕 | 221002, 徐州医学院肿瘤研究所神经生物学研究中心实验室 | | 于红丽 | 221002, 徐州医学院肿瘤研究所神经生物学研究中心实验室 | | 庄明 | 21002, 徐州医学院肿瘤研究所实验动物中心 |
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| Abstract:: |
| Objective To establish a rat model of opening blood-brain barrier with conventional whole-brain irradiation. Methods According to different dosage of irradiation, a hundred Sprague-Dowley rats were randomly assigned into five groups, the control group (no irradiation), and four irradiated groups at 10 grays,20 grays, 30 grays and 40 grays. The rats were administered to conventional fraction irradiation (2 Gy/day and 5 days a week) with routine 60Co gamma-rays. The intake of feed and autonomic activities were observed every day. Changes in skin and hair in the irradiated field, body weight, and center nervous system symptoms and signs were examined and recorded every week during irradiation. The neurological status was ranked on a scale based on the Mickley's Scale. Ultrastructure changes of blood-brain barrier at 16 hours after the last irradiation were examined with electron microscope using lanthanum trace labeling. Results Neither abnormal nervous sign, nor change of feed intake, skin and hair was observed in all the rats. No statistically significant difference of body weight was observed among the five groups (P>0.05). The effect that radiation can directly damage the function and structure of blood-brain barrier was proportional to irradiation doses. Conclusion This rat model is a suitable for study on blood-brain barrier pathophysiology and molecular biology after conventional whole-brain irradiation. ; |
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