| LIU Xia,LI Ping,ZHANG En.Radioprotective effect of c-ski on rat skin fibroblast in vitro[J].Chinese Journal of Radiological Medicine and Protection,2006,26(2):141-143 |
| Radioprotective effect of c-ski on rat skin fibroblast in vitro |
| Received:May 13, 2005 |
| DOI： |
| KeyWords:c-ski Fibroblast Radiation Apoptosis |
| FundProject:国家重点基础研究发展规划资助项目(G1999054205);国家自然科学基金资助项目(30400468) |
| Author Name | Affiliation | E-mail | | LIU Xia | Molecular Biology Center, Research Institute of Surgery, Da Ping Hospital and the Third Military Medical University, 400042 Chongqing, China | ygzhou@cta.cq.cn | | LI Ping | Molecular Biology Center, Research Institute of Surgery, Da Ping Hospital and the Third Military Medical University, 400042 Chongqing, China | | | ZHANG En | Molecular Biology Center, Research Institute of Surgery, Da Ping Hospital and the Third Military Medical University, 400042 Chongqing, China | | | 刘苹 | Molecular Biology Center, Research Institute of Surgery, Da Ping Hospital and the Third Military Medical University, 400042 Chongqing, China | | | 周萍 | Molecular Biology Center, Research Institute of Surgery, Da Ping Hospital and the Third Military Medical University, 400042 Chongqing, China | | | 周元国 | Molecular Biology Center, Research Institute of Surgery, Da Ping Hospital and the Third Military Medical University, 400042 Chongqing, China | |
|
| Hits: 2785 |
| Download times: 2097 |
| Abstract:: |
| Objective To examine radioprotective effect of c-ski on rat skin fibroblast in vitro and explore its possible mechanism. Methods The effect of soft X-ray irradiation at dose varied from 2 to 8 Gy on cell apoptosis in rat skin fibroblast were determined by flow cytometry with Annexin-V-FITC-PI labelling. The effect of c-ski gene transfection on cell apoptosis was evaluated after soft X-ray irradiation of 4 Gy. The protein expressions of Bax and Bcl-2 after c-ski gene transfection were measured with the Western blot method. Results Soft X-ray irradiation increases cell apoptosis, and the increase is proportional to the irradiation dose. Apoptosis ratio increases with time since the irradiation, and reaches its peak at 36h after the irradiation. c-ski gene was observed to markedly decrease apoptosis index at 24 h after soft X-ray irradiation of 4 Gy compared to the control group, significant increase of the protein expression of Bcl-2 was observed. C-ski gene was found no significant effect on the protein expression of Bax. Conclusion c-ski gene can decrease radiation sensitivity of skin fibroblast, promoting Bcl-2 protein expression is one of its possible mechanism for this radioprotective effects. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
