| CHEN Jun,YU Zhi-jian,XIAO Ming-xing.The inhibition effect of P-glycoprotein on cytochrome c release and caspase-3 activation by X-ray irradiation.[J].Chinese Journal of Radiological Medicine and Protection,2006,26(2):118-120 |
| The inhibition effect of P-glycoprotein on cytochrome c release and caspase-3 activation by X-ray irradiation. |
| Received:March 15, 2005 |
| DOI： |
| KeyWords:P-glycoprotein Cytochrome c caspase-3 X-ray |
| FundProject:国家自然科学基金资助项目(30100045) |
| Author Name | Affiliation | | CHEN Jun | Oncology Center, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China | | YU Zhi-jian | Oncology Center, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China | | XIAO Ming-xing | Oncology Center, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China | | 卜俊国 | Oncology Center, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China | | 张积仁 | Oncology Center, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China |
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| Abstract:: |
| Objective To investigate the effects of P-glycoprotein (P-gp) on cytochrome c (Cyt c)release and caspase-3 activation in drug-resistant tumor cell after X-ray irradiation. Methods Anti-P-gp McAb UIC-2 was applied to block P-gp function. MCF-7/Adr, P-gp-overexpressed drug-resistant breast cancer cell line, was irradiated by X-rays. Flow cytometry was used to measure apoptosis, dynamic changes of mitochondrial cytochrome c release and caspase-3 activation at various time after X-ray irradiation. Results Both the expression of cyt c and the activation of caspase-3 in P-gp-blocked group were up-regulated significantly, compared with those in control group at 6 h, 12 h, and 24 h after X-ray irradiation, respectively. Conclusion The current study showed that inhibition of P-gp function can increase cytochrome c release and caspase-3 activation.It suggested that P-gp inhibits X-ray induced cytochrome c release and caspase-3 activation. ; |
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