| YAN Yu-qian,Yan Yu-qiu,WANG Lin.Suppressive effect on HeLa cells proliferation by phenothiazine derivatives alone and combining with ionizing radiation[J].Chinese Journal of Radiological Medicine and Protection,2006,26(2):114-117 |
| Suppressive effect on HeLa cells proliferation by phenothiazine derivatives alone and combining with ionizing radiation |
| Received:April 29, 2005 |
| DOI： |
| KeyWords:Ionizing radiation Phenothialzines Proliferation Synergistic effect |
| FundProject:国家高技术研究发展计划(863)资助项目(2004AA221160);国家自然科学基金资助项目(30270423) |
| Author Name | Affiliation | E-mail | | YAN Yu-qian | Beijing Institute of Radiation Medicine, Beijing 100850, China | zhoupk@nic.bmi.ac.cn | | Yan Yu-qiu | 307 Hospital of China People's Liberation Army | | | WANG Lin | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 彭涛 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 隋建丽 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 白贝 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 孙伟建 | 307 Hospital of China People's Liberation Army | | | 周平坤 | Beijing Institute of Radiation Medicine, Beijing 100850, China | |
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| Abstract:: |
| Objective To examine the antiproliferative effects of phenothialzine derivatives (PTZDs) alone on HeLa cells and in combination with ionizing radiation. Methods MTT and colony-forming method were used to evaluate the proliferation activity and cellular radiosensitivity of HeLa cells. Results We compared the antiproliferative effects of six phenothialzine derivatives, and found that the derivatives α-chloro-N- dimethylamine phenothialzine (PTZD2), α-triflumethyl-N- α dimethylamine ethyl) phenothialzine (PTZD3) and α-chloro-N-(dimethylamine ethyl) phenothialzine (PTZD5) showed a significant antiproliferative effect at concentration of 10 μmol/L. HeLa cells proliferation was completely suppressed when treated with PTZDs of 40-50 μmol/L. PTZD2/PTZD3 and colbat-60 gamma-irradiation showed synergistic suppressive effect on proliferation of HeLa cells. The enhancement ratios of 10 μmol/L PTZD3 combination with 2 Gy and 4 Gy irradiations were 3.5 and 1.8, respectively. The maximum synergistic suppressive effect was observed when cells administered with PTZD3 at 18 h before being irradiated. Conclusion Phenothialzine derivatives show antiproliferations on HeLa cells, and differ in degrees. The synergistic anticancer effect could be obtained by combining phenothialzine derivatives with radiotherapy. |
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