| LONG Xian-hui,XU Qin-zhi,HE Xing-peng.Comparison of transcription profiles between 2 Gy and 10 Gy irradiated human lymphoblastoid cells[J].Chinese Journal of Radiological Medicine and Protection,2006,26(2):110-113 |
| Comparison of transcription profiles between 2 Gy and 10 Gy irradiated human lymphoblastoid cells |
| Received:August 20, 2005 |
| DOI： |
| KeyWords:Gene Expression Signal transduction Cell cycle regulation Microarray assay Ionizing radiation |
| FundProject:国家高技术研究发展计划(863)资助项目(2004AA221160);国家自然科学基金资助项目(30270423) |
| Author Name | Affiliation | E-mail | | LONG Xian-hui | 421001, 湖南衡阳南华大学公共卫生学院 | zhoupk@nic.bmi.ac.cn | | XU Qin-zhi | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | HE Xing-peng | 421001, 湖南衡阳南华大学公共卫生学院 | | | 安静 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 隋建丽 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 白贝 | Beijing Institute of Radiation Medicine, Beijing 100850, China | | | 周平坤 | Beijing Institute of Radiation Medicine, Beijing 100850, China | |
|
| Hits: 2556 |
| Download times: 2069 |
| Abstract:: |
| Objective To compare the alterations of transcriptional profiles between 2 Gy and 10 Gy γ-ray irradiated cells. Methods Human normal lymphoblastoid cell AHH-1 line was irradiated with cobalt-60 γ-rays of 2 Gy or 10 Gy. cDNA microarray,containing the probes corresponding to 14,022 human genes, was used to develop the transcriptional profiles of the cells at 4 h after irradiation. Double microarray was carried out for each dose group, and a 2-fold increase or decrease in signal intensity was treated as significant change in gene expression. Results The microarray assays showed 32 up-regulated genes and 46 down-regulated genes in cells irradiated with 2 Gy γ-rays,while there were 219 genes down-regulated and 26 genes up-regulated in cells irradiated with 10 Gy γ-rays. In addition, it was observed that 11genes were down-regulated and 9 genes up-regulated in both 2 Gy and 10 Gy irradiated groups, most of these altered genes could be related to signal transduction,cell cycle control,DNA repair and apoptosis. Conclusion Same genes were observed to be down-regulated or up-regulated with high or moderate dose irradiation. Ten grays irradiation can depress a great number of genes, which might lead to the failure of multiple cellular biological functions. This study shed light on molecular mechanisms of the diverse biological effects induced by different doses of ionizing radiation. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
