| TIAN Mei,PIAO Chun-ji,LIU Lin-lin,et al.Apoptosis and down-reguled expression of Bcl-2 in SHG-44 glioma cells induced by pEgr-hPTEN stable transfection in combination with X-ray irradiation[J].Chinese Journal of Radiological Medicine and Protection,2006,26(2):106-109 |
| Apoptosis and down-reguled expression of Bcl-2 in SHG-44 glioma cells induced by pEgr-hPTEN stable transfection in combination with X-ray irradiation |
| Received:April 23, 2005 |
| DOI： |
| KeyWords:pEgr-hPTEN X-ray Glioma Apoptosis Bcl-2 |
| FundProject:国家自然科学基金资助项目(30170290) |
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| Abstract:: |
| Objective To investigate the apoptotic effect and the changes of Bcl-2 protein expression of pEgr-hPTEN stable transfection in SHG-44 human glioma cells in combination with irradiation. Methods pEgr-hPTEN vector containing the exogenous wild type PTEN gene was transfected into SHG-44 cells under mediation of lipofectamine in vitro; the positive cell clones called SHG-44-hPTEN were selected and amplified by using G418. Transmission electron-microscope was used to detect the cell ultrastructural changes and flow cytometry to measure the apoptotic effect and Bcl-2 expression of SHG-44- hPTEN cells after X-ray irradiation at different doses. Results Many degenerative changes and early apoptotic changes including the chromosome condensate around the nuclear envelope were observed in SHG-44- hPTEN cells. pEgr-hPTEN stable transfection in combination with X-ray irradiation can significantly induce the apoptosis of SHG-44 cells. The percentage of early apoptosis is of SHG-44-hPTEN cells irradiated with X-rays at different doses was 1.5-2.3 times as much as that of SHG-44-hPTEN/0 Gy group, 1.9-4.4 times as much as that of SHG-44 irradiated group, and 3.4-5.1 times as much as that of SHG-44/0 Gy group. The expression of Bcl-2 proteins decreased in dose-dependent manner. Conclusion PTEN stable transfection in combination with irradiation can significantly induce the apoptosis of tumor cells and significantly down-regulate the expression of Bcl-2 protein. |
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