| WANG Xi-ling,ZHANG Xiang,CAO Rui-feng,et al.Research on inhibitory effect of 60Co γ-irradiation on C6 glioma proliferation and the related molecular mechanism[J].Chinese Journal of Radiological Medicine and Protection,2006,26(1):55-58 |
| Research on inhibitory effect of 60Co γ-irradiation on C6 glioma proliferation and the related molecular mechanism |
| Received:June 06, 2005 |
| DOI： |
| KeyWords:Glioma Apoptosis Cell cycle Flow cytometry p53 p21 |
| FundProject: |
| Author Name | Affiliation | | WANG Xi-ling | Neurosurgical Institute of PLA,Xijing Hospital,Fourth Military Medical University,Xi'an 710032,China | | ZHANG Xiang | Neurosurgical Institute of PLA,Xijing Hospital,Fourth Military Medical University,Xi'an 710032,China | | CAO Rui-feng | Neurosurgical Institute of PLA,Xijing Hospital,Fourth Military Medical University,Xi'an 710032,China | | 曹云新 | 710032 西安,第四军医大学西京医院基础部医学免疫学教研室 | | 梁景文 | Neurosurgical Institute of PLA,Xijing Hospital,Fourth Military Medical University,Xi'an 710032,China | | 马福成 | 710032 西安,第四军医大学西京医院西京医院病理科 | | 边杰芳 | 710032 西安,第四军医大学西京医院血管内分泌科 |
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| Abstract:: |
| Objective The purpose of this study was to observe the effect of 60Co γ-irradiation on C6 rat astrocytoma cells and investigate the possible mechanism.Methods Cultured C6 cells were divided into 4 groups to receive different doses of γ-irradiation: control group, 4 Gy group,16 Gy group,and 64 Gy group.After irradiation the growth curve of cells was made by the methyl thiazolyl tetrazolium（MTT） assay.Flow cytometry was utilized to quantify the percentage of cell apoptosis and the cell cycle distribution 48 h after treatment.P53 and P21 expressions in the cells before and after treatment were analyzed by immunocytochemistry.Results Significant inhibition of proliferation was found in cells of the 16 Gy and 64 Gy groups.As the irradiation doses were raised,the apoptotic rates were significantly increased [3.1±0.5,16.7±0.6,27.3±0.4 and（36.7±0.7）% for the control,4 Gy,16 Gy and 64 Gy groups,respectively,P<0.01].Howerver,the necrotic rates of the four groups were not significantly different（P>0.05）.Meanwhile,as the doses of irradiation were raised,a significantly larger percentage of cells in G0/G1 phase and a smaller one in S phase were found.The expressions of P53 and P21 were both enhanced after irradiation and the enhancement was dose-dependent.Besides,the expressions of P53 and P21 were positively related and the correlation coefficient was 0.889. Conclusion The inhibition of proliferation of C6 cells after 60Co γ-irradiation may be realized simultaneously by cell apoptosis,G1 arrest and S delay.The p53-p21 pathway may be involved in this process. |
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