| CHEN Dao-zhen,LIU Lu,TANG Qiu-sha,et al.In vivo and in vitro experimental study on cervix cancer with combination of HSV-TK/GCV suicide gene therapy system and 60Co radiotherapy[J].Chinese Journal of Radiological Medicine and Protection,2006,26(1):39-42 |
| In vivo and in vitro experimental study on cervix cancer with combination of HSV-TK/GCV suicide gene therapy system and 60Co radiotherapy |
| Received:June 09, 2005 |
| DOI： |
| KeyWords:Herpes simplex virus thymidine kinase(HSV-TK) Cervical cancer Suicide gene therapy Radiotherapy |
| FundProject:国家自然科学基金资助项目(30500601);江苏省科技厅社会发展基金资助项目(BS2003614) |
| Author Name | Affiliation | | CHEN Dao-zhen | Central Laboratory,Wuxi Municipal Hospital for Mother and Child Health Care,Medical School of Nanjin University,Wuxi 214002,China | | LIU Lu | 东南大学医学院 | | TANG Qiu-sha | 东南大学医学院 | | 薛文群 | Central Laboratory,Wuxi Municipal Hospital for Mother and Child Health Care,Medical School of Nanjin University,Wuxi 214002,China | | 詹惠英 | Central Laboratory,Wuxi Municipal Hospital for Mother and Child Health Care,Medical School of Nanjin University,Wuxi 214002,China | | 朱云霞 | Central Laboratory,Wuxi Municipal Hospital for Mother and Child Health Care,Medical School of Nanjin University,Wuxi 214002,China | | 杨幼易 | Central Laboratory,Wuxi Municipal Hospital for Mother and Child Health Care,Medical School of Nanjin University,Wuxi 214002,China |
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| Abstract:: |
| Objective To evaluate the killing effect of HSV-TK/GCV suicide gene therapy system combined with 60Co radiotherapy on human cervical cancer HeLa cell line in vivo and in vitro, and to explore radiosensitization by the HSV-TK/GCV system. Methods The HSV-TK/GCV suicide gene therapy system and 60Co radiotherapy were used separately or in combination for human cervical cancer HeLa cell line in vivo and in vitro to compare their effects.Colony formation test and the rate of radiosensitization effect(E/O)were employed to observed the radiosensitization by the HSV-TK/GCV system. Results The HSV-TK/GCV suicide gene therapy system showed strong therapeutic effects on HeLa cells both in vitro and in vivo(the inhibition rates were 45.8% and 39.5%,respectively).Moreover,the combined application of gene therapy and radiotherapy exhibited stronger therapeutic effects in vitro and in vivo(the inhibition rate was 87.5% in vitro,and was 87.9% in vivo)(P<0.01).The inhibition rate of radiotherapy alone was 42.4% in vitro and 35.8% in vivo.The sensitivity to radiotherapy in combined therapy increased more than that of either therapy alone,and the ability of colony formation decreased(P<0.01).The rate of radiosensitization effect(E/O)was 3.2(>1.4),indicating the HSV-TK/GCV system could exert a sensitizing effect on 60Co radiotherapy of the transplanted human cervical cancer cells in nude mice. Conclusion The HSV-TK/GCV system has radiosensitizationaction.Gene therapy combined with radiotherapy may be a good supplementary method for synthetic treatment of cervical cancer. |
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