| JIA Ting-zhen,LIU Chang-an,YANG Guang,et al.Basic and clinical researches of human cord blood[J].Chinese Journal of Radiological Medicine and Protection,2006,26(1):10-14 |
| Basic and clinical researches of human cord blood |
| Received:September 28, 2005 |
| DOI： |
| KeyWords:Human cord blood Hematopoietic stem/progenitor cells T lymphocytes NK cells Low dose irradiation |
| FundProject: |
| Author Name | Affiliation | | JIA Ting-zhen | The Third Hospital of Peking University,Beijing 100083,China | | LIU Chang-an | The Third Hospital of Peking University,Beijing 100083,China | | YANG Guang | The Third Hospital of Peking University,Beijing 100083,China | | 严智昌 | The Third Hospital of Peking University,Beijing 100083,China | | 陈旭芳 | The Third Hospital of Peking University,Beijing 100083,China | | 李惠平 | The Third Hospital of Peking University,Beijing 100083,China | | 马力文 | The Third Hospital of Peking University,Beijing 100083,China | | 张淑兰 | The Third Hospital of Peking University,Beijing 100083,China | | 王保中 | The Third Hospital of Peking University,Beijing 100083,China |
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| Abstract:: |
| Objective To provide basis for clinical application of cord blood. Methods Using freshly isolated mononuclear cells without or with 62 mGy low dose irradiation by 60Co γ-rays and with 3H-TdR incorporation,release assay,ELISA,flow cytometry,MTT and CFU-GM were performed to study the quantity of cord blood,the number of MNC,active MNC percentage,activities of T cells and NK cell,the expressions of membrane molecules of T lymphocytes,levels of IL-2 TNFα,sIL-2R,cytotoxity of LAK cells aganst target cells（K562,HL-60）,CFU-GM,activity changes of T cells and NK cells after irradiation by low dose γ-rays,activity of colony-stimulating factors,respectively. Results The quantity of cord blood was ranged 100-150 ml,and there was no relation to the MNC number.The cord blood was rich in hematopoietic stem /progenitor cells.Immunological activities of T and NK cells was low,but they were enhanced after low dose γ-ray irradiation.The serum of cord blood was rich in colony-stimulating factors,chiefly IL-3.The clinical application of cord blood for treatment of leukopenia would be obviously effective. Conclusion The prospect for clinical application of human cord blood is encouraging. |
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