| YANG Guang,LIU Chang-an,JIA Ting-zhen.Effect of gamma-ray irradiation on cord blood lymphocyte proliferation and NK cell activity[J].Chinese Journal of Radiological Medicine and Protection,2006,26(1):6-9 |
| Effect of gamma-ray irradiation on cord blood lymphocyte proliferation and NK cell activity |
| Received:September 28, 2005 |
| DOI： |
| KeyWords:Gamma-ray Cord blood Lymphocyte Natural killer cell Proliferation |
| FundProject:国家自然科学基金资助项目(39770238) |
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| Abstract:: |
| Objective To investigate the effects of gamma-ray irradiation on cord blood lymphocyte proliferation and NK cell activity. Methods Freshly isolated mononuclear cells from human cord blood were irradiated with different doses（0.248～15.872 Gy） of gamma-rays.The lymphocyte proliferation and NK cell activity were measured using 3H-TdR incorporation assay and 3H-TdR release assay,respectively. Results In dose range of 0.248～15.872 Gy,lymphocyte proliferation was inhibited and the inhibition rate was positively correlated with the irradiation dose（r=0.839,P<0.05）.Lymphocyte proliferation was not found in dose range of 3.968～15.872 Gy.Irradiation doses from 0.248 to 1.984 Gy could enhance NK cell activity.The activity of NK cells was reserved after irradiated with 3.968 Gy.Within the dose range of 5.952～15.872 Gy,NK cell activity was significantly inhibited. Conclusion Lymphocyte proliferation is inhibited and the activity of NK cell is reserved when irradiated with the dose of 3.968 Gy gamma-rays.So if the lymphocytes are irradiated with such a dose before donor lymphocytes infusion or mix-cord blood transplantation,the effect of graft versus host disease（GVHD） could be decreased whereas the effect of graft versus leukemia（GVL） reaction could be reserved simultaneously during adoptive cellular immunotherapy. |
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