| QIAN Xin,HAN Yun,SUN Yan,et al.Study of the effect of radiation damage on different kinds of cells transfected with HRAD17[J].Chinese Journal of Radiological Medicine and Protection,2006,26(1):3-5 |
| Study of the effect of radiation damage on different kinds of cells transfected with HRAD17 |
| Received:September 28, 2005 |
| DOI： |
| KeyWords:HRAD17 Radiation damage Cell cycle Apoptosis |
| FundProject:国家自然科学基金资助项目(30470976) |
| Author Name | Affiliation | E-mail | | QIAN Xin | The Central Laboratory,Third Hospital of Beijing University,Beijing 100083,China | yingbaozhu@medmail.com.cn | | HAN Yun | The Central Laboratory,Third Hospital of Beijing University,Beijing 100083,China | | | SUN Yan | The Central Laboratory,Third Hospital of Beijing University,Beijing 100083,China | | | 杨业鹏 | 基础医学院放射医学教研室 | | | 朱应葆 | The Central Laboratory,Third Hospital of Beijing University,Beijing 100083,China | |
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| Abstract:: |
| Objective To study the role of HRAD17 in DNA damage and apoptosis induced by ionizing radiation. Methods Plasmids including pDOR-neo vector,pDORHRAD17s（sense） and pDOR-HRAD17a（anti-sense） were transfected into mouse fibroblasts（NIH3T3） and human uterine cervix cancer cells（HeLa）.Positive clones were screened by G418.After 48 h of serume-free culture,all kinds of transfected cells were exposed to 10 Gy γ-rays（at 2.54 Gy/min） and immediately cultured with serum.The cells were isolated and fixed by 70% ethanol,with were collected at 0,6,12,24,36 and 48 h post-irradiation,and stained by propedium iodide（PI） in the dark.Flow cytometry was performed to detect DNA in these cells. Results （Ionizing） radiation could induce G1/S arrest in normal cells transfected with sense HRAD17,but G2/M arrest in tumour cells.Interestingly,cells transfected with antisense HRAD17 showed an elevated apoptotic rate compared to the control group. Conclusion HRAD17 is able to function in both G1/S and G2/M arrest and inhibit apoptosis induced by radiation damage. |
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