| ZHOU Li-xia,ZHANG Ming-feng,YANG Tian-zhu,et al.TNF-α and IL-1β expression changes in brain and peripheral blood after gamma knife treatment for rats with brain glioma[J].Chinese Journal of Radiological Medicine and Protection,2005,25(6):539-542 |
| TNF-α and IL-1β expression changes in brain and peripheral blood after gamma knife treatment for rats with brain glioma |
| Received:December 15, 2004 |
| DOI： |
| KeyWords:Glioma Gamma knife Tumor necrosis factor-α Interleukin-1β(IL-1β) Hybridization in situ |
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| Abstract:: |
| Objective By observing the expressions of the TNF-α and IL-1β in the brain of rats with glioma after gamma knife(GK) therapy, to investigate the role of neuroimmunomodulation in the stereotaxic radiosurgery treatment. Methods A total of 20 normal adult male SD rats were divided into 4 groups: the normal control group (group N), normal rats with GK treatment group (group NR), tumor group (group T) and GK treatment for the tumor group (group TR). On day 14 after implantation of glioma cells, the rats in groups T and N were sacrificed and their brains were sampled, whereas the rats in groups TR and NR were undergone the GK irradiation with the same procedure mentioned above. On day 14 after irradiation, all of the rats were examined with in situ hybridization for TNF-α and IL-1β mRNA in the brain tissue, and the content of TNF-α in the peripheral blood was also determined. Results In groups N and NR, minimal levels of TNF-α and IL-1β mRNA could be detected in the brain, and a small amount of weak positive cells were dispersed in some areas such as the cerebral cortex, some hypothalamic nuclei, etc. The area density of the positive cells in the brain of the group T was higher than that of groups N and NR (P<0.01), and a large amount of infiltrating glial cells and monocyts were found in the tumour area. The area density of positive cells in the brain of the group TR was higher than that of group T(P<0.01); (2) ANONA test showed that the contents of TNF-α and IL-1β in the peripheral blood of group TR and group T were significantly higher than those in groups N and NR, and those of group TR were higher than those of group T(P<0.01). Conclusions After GK treatment, the levels of TNF-α and IL-1β mRNA increased in the brain of rats with glioma, and the TNF-α and IL-1β contents in the peripheral blood of the rats were also increased, suggesting that the changes might be related to the changes of the immune status induced by the gamma knife treatment. |
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