| LIU Ai-guo,YANG Mo,LI Zhi-guang,et al.Protective effect of melatonin on thrombocytopoiesis in irratiated mice[J].Chinese Journal of Radiological Medicine and Protection,2005,25(6):536-539 |
| Protective effect of melatonin on thrombocytopoiesis in irratiated mice |
| Received:February 04, 2005 |
| DOI： |
| KeyWords:Melatonin Megakaryocyte(MK) CFU-MK Thrombocytopoiesis(T) |
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| Abstract:: |
| Objective To study the protective effect of melatonin on thrombocytopoiesis(T) and its mechanism in total-bodily irradiated mice. Methods Altogether 18 female BALB/c mice were randomly divided into three experimental groups (6 each): Group 1(normal control, N) received neither irradiation nor melatonin; Group 2 (model control, C); received total body-irradiation for 4 Gy gama-rays and Group 3(melatonin, M), received melatonin after irradiation at the dosage of 10 mg·kg-1 ·d-1 via i.p.injection in consecutive 21 days. In Group C normal saline instead of melatonin was administered in the same way as above. Peripheral blood platelets and white blood cells(WBC) were analyzed for the three groups on day 0,day 7,day 14, and day 21. All the mice were sacrificed to collect bone marrow cells for the assays of colony-forming unit-megakaryocyte (CFU-MK) and of colony-forming unit-fibroblast (CFU-F). The effects of melatonin of different concentrations (0-500 nmol/L) on CFU-MK formation were observed in vitro. Results The results showed that melatonin enhanced the recovery of T. Moreover, melatonin also promoted the increase of CFU-F (28±10.4 vs 14.6±2.8) and CFU-MK (19.63±3.28 vs 11±2.24) in vivo. The amount of CFU-MK in vitro was dependent on the concentration of melatonin. Compared with the control group, the size of CFU-MK in Group M was much larger and MK cells were more mature, especially when the melatonin concentration was 200 nmol/L. Conclusion Melatonin provides protective effect on T in irradiated mice. It enhances T in vivo and promotes the growth of bone marrow stromal cells as well as megakaryocytes in vitro. Therefore, we speculate that the T-protective activity of melatonin may be mediated via promoting growth of the progenitors of platelet, megakaryocytes, and bone marrow stromal cells. |
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