| LI Yong,JIN Ning,YANG Hai-shan.The effect of endostatin gene in combination with radiotherapy on rats with implanted tumor[J].Chinese Journal of Radiological Medicine and Protection,2005,25(6):531-533 |
| The effect of endostatin gene in combination with radiotherapy on rats with implanted tumor |
| Received:March 31, 2005 |
| DOI： |
| KeyWords:Walker-256 tumor cell Gene therapy Radiotherapy |
| FundProject:吉林省科技厅重点基金资助项目(20050335) |
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| Abstract:: |
| Objective To study the effect of combinating of the radiotherapy with endostatin gene therapy on the rats with implanted tumor. Methods Immediate Walker-256 cancerous ascetic injection method was used to make a rat tumor-bearing model, then the tumor was treated with saline,endostatin gene,irradiation or endostatin gene plus irradiation. The tumor growth rate and weight were observed, Western blot and RT-PCR were adopted to check the expressions of endostatin mRNA and protein. Results The expressions of endostatin mRNA and protein were significant in the gene therapy group and the gene plus radiotherapy group, but there was a significant difference between these two groups. As compared with the control group, the tumor growth rate and weight decreased significantly in all the therapy groups (P<0.001). The effect of gene plus radiotherapy on the implanted tumor was the best among all groups. There was no significant difference in therapeutic effect between the gene therapy group and the radiation therapy group (P>0.05). Conclusion After the pCMV-Endostatin was induced, the expressions of endostatin mRNA and protein was significant in Walker-256 tumor and the tumor growth was inhibited. However,the effect of the endostatin gene plus radiotherapy was obviously better than that of the endostatin gene therapy group or the radiotherapy group for inhibiting tumor growth.; |
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