| FAN Yan,XU Ai-hua,XU Lan.Effects of β-rays on the expressions of MMP2 and PPGalNAcT2 in NIH3T3 cell lines and the interference of Taurine[J].Chinese Journal of Radiological Medicine and Protection,2005,25(6):528-530 |
| Effects of β-rays on the expressions of MMP2 and PPGalNAcT2 in NIH3T3 cell lines and the interference of Taurine |
| Received:February 21, 2005 |
| DOI： |
| KeyWords:β-irradiation Matrix metalloproteinase2 (MMP2) Polypeptide N-acetylgalactosaminyltransferase 2 (ppGalNAcT2) Taurine (Tau) |
| FundProject:国防科工委国防基础研究资助项目(2003044);江苏省高校自然科学基金资助项目(01KJB310001) |
| Author Name | Affiliation | E-mail | | FAN Yan | Department of Biochemistry and Molecular Biology, Medical College of Soochow University, Suzhou 215007, China | shiliangwu@hotmail.com | | XU Ai-hua | Department of Biochemistry and Molecular Biology, Medical College of Soochow University, Suzhou 215007, China | | | XU Lan | Department of Biochemistry and Molecular Biology, Medical College of Soochow University, Suzhou 215007, China | | | 仇灏 | Department of Biochemistry and Molecular Biology, Medical College of Soochow University, Suzhou 215007, China | | | 吴士良 | Department of Biochemistry and Molecular Biology, Medical College of Soochow University, Suzhou 215007, China | |
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| Abstract:: |
| Objective To observe the effects of β-irradiation on the mRNA expressions of MMP2(matrix metalloproteinase 2) and ppGalNAcT2 (polypeptide N-acetylgalactosaminyltransferase 2) in NIH3T3 cells, and to study the biologic protective effect of Tau(Taurine) for β-irradiation’s damage. Method After NIH3T3 cells (including Tau groups)were exposed to different doses of high energy electronic rays which mocked β-irradiation, the expressions at mRNA level of MMP2 and ppGalNAcT2 were examined by reverse transcription polymerase chain reaction (RT-PCR) within 12 h. Results The results showed that the mRNA expression of MMP2 was increased evidently in NIH3T3 cells irradiated by β-irradiation, and it was positively related to the dosage. The changes of ppGalNAcT2 was Tau could interfere with the changes.The mRNA expression of ppGalNAcT2 in NIH3T3 cells could be inhibited by β-irradiation,and it is postulated that ppGalNAcT2 might be related to the progression of cell’s dissociation. Conclusion Tau can inhibit the expressions of MMP2, meanwhile stimulate the expression of ppGalNAcT2, so it has biologic protection effect at cell level. |
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