| WANG Chun-yan,ZHANG Wei,SU Xu.Okadaic acid for radiation dose estimation using drug-induced premature chromosome condensation[J].Chinese Journal of Radiological Medicine and Protection,2005,25(6):526-527 |
| Okadaic acid for radiation dose estimation using drug-induced premature chromosome condensation |
| Received:July 25, 2005 |
| DOI： |
| KeyWords:Radiation Biodosimetry Peripheral blood lymphocytes Premature chromosomes condensedation |
| FundProject:北京市自然科学基金资助项目(7032043号);科技部社会公益研究专项重点项目(2003DIA6N009) |
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| Abstract:: |
| Objective To establish simple biological method for high irradiation dose estimation using drug-induced prematurely condensd chromosomes(PCC) aberrations. Methods Peripheral blood was taken from healthy adults and irradiated by 0, 1, 2, 5, 10, 15, 20 and 25 Gy 60Co γ-rays. Then the blood was samples were cultured for 48 hrs. One hr before the end of culture, okadaic acid was added into culture medium to induce PCC rings,which were counted for each dose point. Results The yield of PCC rings was increased with the dose of radiation until 20 Gy. Within the range of 1 to 20 Gy, there was a good dose-response relationship between the yield of PCC rings and radiation dose. Conclusion Compared with the analysis of frequency of dicentrics, the yield of PCC rings could be a good biodosimetry indicator for estimation of high dose irradiation. |
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